Woods G T, Watrach A M, Feorino P, Zinzilieta M
Can J Comp Med. 1970 Apr;34(2):122-5.
Three viral agents isolated from nasal exudate of Illinois cattle affected with respiratory disease were studied. The agents were classified as picornaviruses on the basis of their resistance to ether, stability at pH 3.0, and morphological characteristics. Magnesium chloride at 1 M concentration protected the viruses from reduction of infectivity by exposure to 50 degrees C for one and two hours. None of the viruses was pathogenic for mice. Replication of one of the viruses was not affected by the DNA inhibitor, 5 fluorodeoxyuridine. That two of the agents were closely related serologically was demonstrated by serum neutralization tests. As revealed by electron microscopy, viral particles were spherical or hexagonal and ranged in size from 280 A to 290 A.
对从患有呼吸道疾病的伊利诺伊州牛的鼻分泌物中分离出的三种病毒制剂进行了研究。根据它们对乙醚的抗性、在pH 3.0下的稳定性和形态特征，这些制剂被归类为微小核糖核酸病毒。1 M浓度的氯化镁可保护病毒免受因暴露于50摄氏度1小时和2小时而导致的感染性降低。这些病毒对小鼠均无致病性。其中一种病毒的复制不受DNA抑制剂5-氟脱氧尿苷的影响。血清中和试验表明，其中两种制剂在血清学上密切相关。电子显微镜显示，病毒颗粒呈球形或六边形，大小在280埃至290埃之间。
