Strott C A, Bermudez J A, Lipsett M B
J Clin Invest. 1970 Nov;49(11):1999-2007. doi: 10.1172/JCI106420.
A reliable radio-ligand assay has been developed for the measurement of 17-hydroxypregnenolone in human peripheral vein plasma. The mean plasma concentration of 17-hydroxypregnenolone was, in men, 1.9 mmug/ml; and in women, 3.5 mmug/ml. These means were not significantly different from each other, and the levels were the same in the follicular and luteal phases of the menstrual cycle. In women, the adrenal cortex was the source of the 17-hydroxypregnenolone; in men, 40% was produced by the testis. Since the metabolic clearance rate was about 2000 liters/24 hr production rate estimates were 4-7 mg/24 hr. The conversion of blood 17-hydroxypregnenolone to blood 17-hydroxyprogesterone and dehydroepiandrosterone was measured. This varied from 5 to 20%. Thus, in women during the follicular phase, 17-hydroxyprogesterone derived from blood 17-hydroxypregnenolone could be the major fraction of the 17-hydroxyprogesterone production rate. Blood 17-hydroxypregnenolone is a minor precursor of blood dehydroepiandrosterone.
已开发出一种可靠的放射配体分析法，用于测定人外周静脉血浆中的17-羟孕烯醇酮。男性血浆中17-羟孕烯醇酮的平均浓度为1.9微克/毫升；女性为3.5微克/毫升。这些平均值彼此无显著差异，且在月经周期的卵泡期和黄体期水平相同。在女性中，肾上腺皮质是17-羟孕烯醇酮的来源；在男性中，40%由睾丸产生。由于代谢清除率约为2000升/24小时，生成率估计为4 - 7毫克/24小时。测定了血液中17-羟孕烯醇酮向血液中17-羟孕酮和脱氢表雄酮的转化率。该转化率在5%至20%之间变化。因此，在卵泡期的女性中，由血液中17-羟孕烯醇酮衍生而来的17-羟孕酮可能是17-羟孕酮生成率的主要部分。血液中的17-羟孕烯醇酮是血液中脱氢表雄酮的次要前体。
