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鸡血清蛋白的放射免疫扩散法。I. 最佳检测程序的标准化

Radial immunodiffusion of chicken serum proteins. I. Standardization of optimal test procedures.

作者信息

Cho H C, Kramer T T

出版信息

Can J Comp Med. 1970 Oct;34(4):341-6.

PMID:4249097
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1319477/
Abstract

Optimal conditions were determined for the quantitaion of chicken serum albumin, conalbumin, IgG and IgM by the radial immunodiffusion test. The best diluent was 0.15 M phosphate buffered saline, pH 7.2. The optimal concentration of the rabbit antiserum in the agar plate was inversely related to the molecular weight of the protein under study. The incubation time required for maximum ring formation was directly related to the molecular weight of the proteins under study. The reproducibility of the tests was evaluated using stored and fresh antiserum-agar plates.

摘要

通过放射免疫扩散试验确定了定量鸡血清白蛋白、伴白蛋白、免疫球蛋白G和免疫球蛋白M的最佳条件。最佳稀释剂是0.15M磷酸盐缓冲盐水,pH值为7.2。琼脂平板中兔抗血清的最佳浓度与所研究蛋白质的分子量呈负相关。形成最大沉淀环所需的孵育时间与所研究蛋白质的分子量直接相关。使用储存的和新鲜的抗血清-琼脂平板评估了试验的可重复性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b5/1319477/8d95e11fe2e1/compmed00064-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b5/1319477/97717e28359a/compmed00064-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b5/1319477/feb3c43c7c1b/compmed00064-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b5/1319477/8d95e11fe2e1/compmed00064-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b5/1319477/97717e28359a/compmed00064-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b5/1319477/feb3c43c7c1b/compmed00064-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b5/1319477/8d95e11fe2e1/compmed00064-0071-a.jpg

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引用本文的文献

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本文引用的文献

1
Fowl antibody: VI. The fixation of complement by antibody to soluble antigens. The effect of fresh normal fowl serum on the precipitation reaction.禽类抗体:VI. 可溶性抗原抗体对补体的固定作用。新鲜正常禽血清对沉淀反应的影响。
Immunology. 1962 Nov;5(6):656-65.
2
Demonstration of a normal serum macroglobulin coprecipitating with the bovine serum albumin (BSA)-chicken anti-BSA precipitate.正常血清巨球蛋白与牛血清白蛋白(BSA)-鸡抗BSA沉淀物共沉淀的证明。
J Immunol. 1960 Nov;85:439-46.
3
Binding properties of purified chicken antibody.纯化鸡抗体的结合特性。
Immunochemistry. 1969 Jul;6(4):573-85. doi: 10.1016/0019-2791(69)90197-9.
4
Thin agarose gel immunologic methods.薄琼脂糖凝胶免疫方法。
Am J Clin Pathol. 1969 Jun;51(6):769-76. doi: 10.1093/ajcp/51.6.769.
5
Immunochemical quantitation of antigens by single radial immunodiffusion.通过单向辐射免疫扩散法对抗原进行免疫化学定量。
Immunochemistry. 1965 Sep;2(3):235-54. doi: 10.1016/0019-2791(65)90004-2.
6
Quantitative estimation of human immunoglobulins. I. Estimation of IGG, IGA and IGM immunoglobulins by the method of simple radial immunodiffusion.人免疫球蛋白的定量测定。I. 用简单单向免疫扩散法测定IgG、IgA和IgM免疫球蛋白
J Hyg Epidemiol Microbiol Immunol. 1969;13(1):107-17.
7
Transfer of immunoglobulins and antibodies in the hen's egg.母鸡卵中免疫球蛋白和抗体的转移。
Immunology. 1970 Jul;19(1):157-67.