胸苷和5-氟尿嘧啶2'-脱氧核苷对培养的P815Y肥大细胞生物合成事件的不同影响。
Different effects of thymidine and 5-fluorouracil 2'-deoxyriboside on biosynthetic events in cultured P815Y mast cells.
作者信息
Bergeron J J
出版信息
Biochem J. 1971 Jul;123(3):385-90. doi: 10.1042/bj1230385.
Abstract
- Addition of 2mm-thymidine, although resulting in the cessation of cell division, allows the continuation of phospholipid and protein synthesis and results in an increase in mean cell volume for at least 15h. 2. 5-Fluorouracil 2'-deoxyriboside inhibits cell division but differs from thymidine by inhibiting the synthesis of phospholipid and protein in a more marked manner. 3. The relation between these results and the P815Y cell cycle is discussed.
摘要
- 添加2毫米胸苷，虽然会导致细胞分裂停止，但能使磷脂和蛋白质合成继续进行，并使平均细胞体积至少在15小时内增加。2. 5-氟尿嘧啶2'-脱氧核糖苷抑制细胞分裂，但与胸苷不同，它更显著地抑制磷脂和蛋白质的合成。3. 讨论了这些结果与P815Y细胞周期之间的关系。
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2
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3
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