一种在酶系统中具有活性的三磷酸腺苷荧光修饰物：1,N⁶-乙烯基三磷酸腺苷。
A fluorescent modification of adenosine triphosphate with activity in enzyme systems: 1,N 6 -ethenoadenosine triphosphate.
作者信息
Secrist J A, Barrio J R, Leonard N J
出版信息
Science. 1972 Feb 11;175(4022):646-7. doi: 10.1126/science.175.4022.646.
PMID:4257930
Abstract
A new, highly fluorescent adenosine triphophate (ATP) analog, 1,N(6) ethenoadenosine triphosphate, has been synthesized. Its fluorescence properties, including the long fluorescence lifetime and the possibility of detection at very low concentrations, in conjunction with its activity in the representative enzyme systems here reported, make it a valuable probe of enzymic mechanism and structure.
摘要
一种新的、具有高荧光性的三磷酸腺苷（ATP）类似物，即1,N(6)-乙烯基三磷酸腺苷，已被合成。它的荧光特性，包括长荧光寿命以及在极低浓度下进行检测的可能性，再结合此处报道的其在代表性酶系统中的活性，使其成为研究酶作用机制和结构的一种有价值的探针。
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