Effects of cholecystokinin-pancreozymin on amylase synthesis and secretion in the mouse pancreas.

Perfusion of mouse pancreatic slices revealed that continuous exposition with cholecystokinin-pancreazymin (CCK-PZ) provokes an initially high peak of amylase secretion, which is followed by a slow decline in enzyme discharge. A return to the basal secretory level is noticed after about 45 min. Repeated pulse stimulations with CCK-PZ result in a more efficient stimulation of pancreatic amylase release, compared to a continuous stimulation. In the presence of CCK-PZ for 45 min, as well as during a post-stimulatory period of 45 min, a significant depression of L-(U-14C) leucine incorporation into amylase as well as total protein is recorded. In conclusion, in vitro incubated mouse pancreatic slices thus seem to be unable to increase their rate of protein synthesis during and after stimulation of secretion.