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用于粉碎和匀化指甲样本的实验室磨具,辅助甲真菌病的显微镜检查和培养确诊。

Laboratory mill for pulverizing and homogenizing nail specimens as an aid to microscopy and culture confirmation of onychomycosis.

作者信息

Luedemann G M, LeBreton E

出版信息

Appl Microbiol. 1972 Apr;23(4):814-8. doi: 10.1128/am.23.4.814-818.1972.

DOI:10.1128/am.23.4.814-818.1972
PMID:4259708
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC380442/
Abstract

A small mill has been developed for reducing nail clippings to a convenient size for microscopy and inoculation onto isolation media. The milling process acts to pulverize and homogenize the specimen. The use of a homogeneous sample in microscopy increases the opportunity for the discovery of fungal hyphae in a specimen. The use of a pulverized specimen increases the possibility of culture isolation by permitting greater numbers of potentially viable fungal cells to come into direct contact with the isolation medium.

摘要

已开发出一种小型研磨机,用于将指甲剪屑研磨至便于显微镜检查和接种到分离培养基上的尺寸。研磨过程可将标本粉碎并使其均匀化。在显微镜检查中使用均匀的样本增加了在标本中发现真菌菌丝的机会。使用粉碎后的标本通过使更多潜在存活的真菌细胞直接接触分离培养基,增加了培养分离的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/31b45a662bb7/applmicro00045-0169-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/dee3beb59922/applmicro00045-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/09038cd91548/applmicro00045-0167-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/f938bb158c81/applmicro00045-0167-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/4ea6e54a6b0c/applmicro00045-0167-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/68027df72213/applmicro00045-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/1b498b0932ac/applmicro00045-0169-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/ed915628c799/applmicro00045-0169-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/87f503b9fb8a/applmicro00045-0169-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/d2971ebb4466/applmicro00045-0169-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/31b45a662bb7/applmicro00045-0169-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/dee3beb59922/applmicro00045-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/09038cd91548/applmicro00045-0167-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/f938bb158c81/applmicro00045-0167-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/4ea6e54a6b0c/applmicro00045-0167-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/68027df72213/applmicro00045-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/1b498b0932ac/applmicro00045-0169-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/ed915628c799/applmicro00045-0169-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/87f503b9fb8a/applmicro00045-0169-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/d2971ebb4466/applmicro00045-0169-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bbe5/380442/31b45a662bb7/applmicro00045-0169-f.jpg

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Laboratory mill for pulverizing and homogenizing nail specimens as an aid to microscopy and culture confirmation of onychomycosis.用于粉碎和匀化指甲样本的实验室磨具,辅助甲真菌病的显微镜检查和培养确诊。
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本文引用的文献

1
Fungi in toe nails.脚趾甲中的真菌。
J Invest Dermatol. 1969 Aug;53(2):140-2.
2
Psoriasis of the nail. A clinical-pathologic study.
Arch Dermatol. 1969 May;99(5):567-79.
3
Evaluation of two new color indicator media for diagnosis of dermatophytosis.两种新型颜色指示培养基用于皮肤癣菌病诊断的评估
Arch Dermatol. 1970 Jul;102(1):68-70.
4
Media with pH indicators for the isolation of dermatophytes.含有pH指示剂用于分离皮肤癣菌的培养基。
Arch Dermatol. 1970 Nov;102(5):545-7.
5
Efficacy of "dermatophyte test medium". Comparison of two commercial preparations with laboratory-prepared Sabouraud-antibiotic medium.“皮肤癣菌测试培养基”的功效。两种市售制剂与实验室自制沙氏抗生素培养基的比较。
Arch Dermatol. 1971 Nov;104(5):486-9. doi: 10.1001/archderm.104.5.486.
6
Onychomycosis caused by fusarium oxysporum.由尖孢镰刀菌引起的甲癣。
Australas J Dermatol. 1971 Apr;12(1):18-29. doi: 10.1111/j.1440-0960.1971.tb00688.x.
7
Superficial white onychomycosis.浅表白色甲真菌病
Sabouraudia. 1966 Oct;5(2):99-103. doi: 10.1080/00362176785190181.