Luedemann G M, LeBreton E
Appl Microbiol. 1972 Apr;23(4):814-8. doi: 10.1128/am.23.4.814-818.1972.
A small mill has been developed for reducing nail clippings to a convenient size for microscopy and inoculation onto isolation media. The milling process acts to pulverize and homogenize the specimen. The use of a homogeneous sample in microscopy increases the opportunity for the discovery of fungal hyphae in a specimen. The use of a pulverized specimen increases the possibility of culture isolation by permitting greater numbers of potentially viable fungal cells to come into direct contact with the isolation medium.
已开发出一种小型研磨机,用于将指甲剪屑研磨至便于显微镜检查和接种到分离培养基上的尺寸。研磨过程可将标本粉碎并使其均匀化。在显微镜检查中使用均匀的样本增加了在标本中发现真菌菌丝的机会。使用粉碎后的标本通过使更多潜在存活的真菌细胞直接接触分离培养基,增加了培养分离的可能性。