Maynard P V, Cameron E H
Biochem J. 1972 Jan;126(1):99-106.
C(19)-steroid metabolism in homogenates of adrenal tissue from rats and mice has been studied. Production of these compounds from [7alpha-(3)H]cholesterol by rat adrenal tissue appeared to follow a route independent of pregnenolone. The major products of [7alpha-(3)H]-dehydroepiandrosterone metabolism by rat adrenal tissue were 5alpha-reduced steroids, principally androsterone, epiandrosterone and 5alpha-androstanedione. No differences in metabolism of [7alpha-(3)H]dehydroepiandrosterone or [4-(14)C]pregnenolone were detected between adrenal tissue from Sprague-Dawley, Wistar and Osborne-Mendel rats, but experiments with the Snell rat adrenocortical tumour 494 showed that this tissue had low 5alpha-reductase activity. In contrast, the major products of [7alpha-(3)H]dehydroepiandrosterone metabolism by mouse adrenal tissue were 5beta-reduced steroids. Differences were observed between LACA and NH strains of mice in that there was a lower metabolism of androstenedione by NH mouse adrenal and a considerable difference in the proportions of aetiocholanolone and epiaetiocholanolone produced.
对大鼠和小鼠肾上腺组织匀浆中的C(19)-类固醇代谢进行了研究。大鼠肾上腺组织由[7α-(3)H]胆固醇生成这些化合物的途径似乎独立于孕烯醇酮。大鼠肾上腺组织对[7α-(3)H]-脱氢表雄酮代谢的主要产物是5α-还原类固醇，主要为雄酮、表雄酮和5α-雄烷二酮。在斯普拉格-道利大鼠、Wistar大鼠和奥斯本-孟德尔大鼠的肾上腺组织中，未检测到[7α-(3)H]脱氢表雄酮或[4-(14)C]孕烯醇酮代谢的差异，但对斯奈尔大鼠肾上腺皮质肿瘤494的实验表明，该组织的5α-还原酶活性较低。相比之下，小鼠肾上腺组织对[7α-(3)H]脱氢表雄酮代谢的主要产物是5β-还原类固醇。在LACA和NH品系小鼠之间观察到差异，即NH小鼠肾上腺对雄烯二酮的代谢较低，且产生的本胆烷醇酮和表本胆烷醇酮比例存在显著差异。
