Flawiá M M, Torres H N
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2870-3. doi: 10.1073/pnas.69.10.2870.
Membrane-bound adenylate cyclase in Neurospora crassa is activated by glucagon. Half-maximal effect is observed at hormone concentrations of about 10 nM. After solubilization of the enzyme with Lubrol-PX, the glucagon effect is lost. Incubation of neurospora cells with glucagon leads to a decrease in the activity of glycogen synthetase (EC 2.4.1.11) and to an increase in the activity of glycogen phosphorylase (EC 2.4.1.1) and in the rate of glycogenolysis.
粗糙脉孢菌中的膜结合腺苷酸环化酶可被胰高血糖素激活。在激素浓度约为10 nM时观察到半数最大效应。用Lubrol-PX溶解该酶后,胰高血糖素的作用消失。用胰高血糖素孵育脉孢菌细胞会导致糖原合成酶(EC 2.4.1.11)活性降低,糖原磷酸化酶(EC 2.4.1.1)活性增加以及糖原分解速率加快。