大肠杆菌镁离子 - 三磷酸腺苷酶缺陷型突变体中主动运输的恢复
Restoration of active transport in an Mg2+-adenosine triphosphatase-deficient mutant of Escherichia coli.
作者信息
Rosen B P
出版信息
J Bacteriol. 1973 Dec;116(3):1124-9. doi: 10.1128/jb.116.3.1124-1129.1973.
Abstract
A neomycin-resistant mutant of Escherichia coli, NR70, lacking membrane-bound Mg(2+)-adenosine triphosphatase (EC 3.6.1.3) activity has been isolated. Both whole cells and membrane vesicles exhibit a reduced ability to accumulate amino acids and sugars. Other membrane-related functions such as oxygen consumption, the in vivo hydrolysis of o-nitrophenyl-beta-d-galactoside, and the phosphoenolpyruvate-dependent phosphotransferase system did not exhibit reduced activities in NR70. Amino acid transport could be partially restored by the addition of N,N'-dicyclohexylcarbodiimide. The results suggest that a role of the Mg(2+)-adenosine triphosphatase may be to participate in the coupling of energy derived from the electron transport chain to other processes such as transport.
摘要
已分离出大肠杆菌的一种新霉素抗性突变体NR70，其缺乏膜结合的Mg(2+)-三磷酸腺苷酶（EC 3.6.1.3）活性。完整细胞和膜囊泡积累氨基酸和糖的能力均降低。其他与膜相关的功能，如耗氧量、邻硝基苯基-β-D-半乳糖苷的体内水解以及磷酸烯醇丙酮酸依赖性磷酸转移酶系统，在NR70中并未表现出活性降低。添加N,N'-二环己基碳二亚胺可部分恢复氨基酸转运。结果表明，Mg(2+)-三磷酸腺苷酶的作用可能是参与将电子传递链产生的能量与其他过程（如转运）偶联起来。
相似文献
1
Restoration of active transport in an Mg2+-adenosine triphosphatase-deficient mutant of Escherichia coli.大肠杆菌镁离子 - 三磷酸腺苷酶缺陷型突变体中主动运输的恢复
J Bacteriol. 1973 Dec;116(3):1124-9. doi: 10.1128/jb.116.3.1124-1129.1973.
2
Impairment and restoration of the energized state in membrane vesicles of a mutant of Escherichia coli lacking adenosine triphosphatase.缺乏三磷酸腺苷酶的大肠杆菌突变体膜囊泡中激发态的损伤与恢复
J Biol Chem. 1974 Jul 25;249(14):4587-93.
3
Physiological suppression of a transport defect in Escherichia coli mutants deficient in Ca2+, Mg2+-stimulated adenosine triphosphatase.大肠杆菌中缺乏Ca2+、Mg2+刺激的三磷酸腺苷酶的突变体中转运缺陷的生理抑制。
J Bacteriol. 1975 Dec;124(3):1248-55. doi: 10.1128/jb.124.3.1248-1255.1975.
4
Effect of dicyclohexylcarbodiimide on growth and membrane-mediated processes in wild type and heptose-deficient mutants of Escherichia coli K-12.二环己基碳二亚胺对大肠杆菌K-12野生型和庚糖缺陷型突变体生长及膜介导过程的影响
J Bacteriol. 1974 Jul;119(1):129-37. doi: 10.1128/jb.119.1.129-137.1974.
5
Use of neomycin in the isolation of mutants blocked in energy conservation in Escherichia coli.新霉素在分离大肠杆菌中能量守恒受阻突变体中的应用。
J Bacteriol. 1972 Jul;111(1):287-9. doi: 10.1128/jb.111.1.287-289.1972.
6
Mutant of Escherichia coli defective in response to colicin K and in active transport.对大肠杆菌素K反应缺陷且主动运输功能缺失的大肠杆菌突变体。
J Bacteriol. 1976 Feb;125(2):467-74. doi: 10.1128/jb.125.2.467-474.1976.
7
Energy transduction in Escherichia coli. The role of the Mg2+ATPase.大肠杆菌中的能量转导。Mg2+ATP酶的作用。
J Biol Chem. 1975 Nov 10;250(21):8409-15.
8
Mechanisms of active transport in isolated bacterial membrane vesicles. XII. Active transport by a mutant of Escherichia coli uncoupled for oxidative phosphorylation.离体细菌膜泡中主动运输的机制。十二、大肠杆菌氧化磷酸化解偶联突变体的主动运输
Arch Biochem Biophys. 1973 Feb;154(2):575-82. doi: 10.1016/0003-9861(73)90011-8.
9
The role of the carbodiimide-reactive component of the adenosine-5'-triphosphatase complex in the proton permeability of Escherichia coli membrane vesicles.腺苷-5'-三磷酸酶复合物中碳二亚胺反应性成分在大肠杆菌膜泡质子通透性中的作用。
Biochemistry. 1976 Jun 29;15(13):2741-6. doi: 10.1021/bi00658a005.
10
Energy transduction in Escherichia coli. Genetic alteration of a membrane polypeptide of the (Ca2+,Mg2+)-ATPase.大肠杆菌中的能量转换。（Ca2 +，Mg2 +）-ATP酶膜多肽的基因改变。
J Biol Chem. 1975 Dec 25;250(24):9421-7.
引用本文的文献
1
Understanding structure, function, and mutations in the mitochondrial ATP synthase.了解线粒体ATP合酶的结构、功能及突变。
Microb Cell. 2015 Apr 1;2(4):105-125. doi: 10.15698/mic2015.04.197.
2
The topology of the proton translocating F0 component of the ATP synthase from E. coli K12: studies with proteases.来自大肠杆菌K12的ATP合酶质子转运F0组分的拓扑结构：蛋白酶研究
EMBO J. 1983;2(1):105-10. doi: 10.1002/j.1460-2075.1983.tb01389.x.
3
Partial assembly of the yeast mitochondrial ATP synthase.酵母线粒体ATP合酶的部分组装
J Bioenerg Biomembr. 2000 Aug;32(4):391-400. doi: 10.1023/a:1005532104617.
4
Bacillus subtilis F0F1 ATPase: DNA sequence of the atp operon and characterization of atp mutants.枯草芽孢杆菌F0F1 ATP合酶：atp操纵子的DNA序列及atp突变体的特性分析
J Bacteriol. 1994 Nov;176(22):6802-11. doi: 10.1128/jb.176.22.6802-6811.1994.
5
Gentamicin uptake in wild-type and aminoglycoside-resistant small-colony mutants of Staphylococcus aureus.庆大霉素在金黄色葡萄球菌野生型和耐氨基糖苷类小菌落突变体中的摄取情况。
Antimicrob Agents Chemother. 1980 Nov;18(5):722-9. doi: 10.1128/AAC.18.5.722.
6
Active transport of amino acids in Thiobacillus thioparus is a low-affinity process.嗜硫硫杆菌中氨基酸的主动转运是一个低亲和力过程。
J Bacteriol. 1981 Dec;148(3):956-65. doi: 10.1128/jb.148.3.956-965.1981.
7
Interaction between aminoglycoside uptake and ribosomal resistance mutations.氨基糖苷类药物摄取与核糖体耐药性突变之间的相互作用。
Antimicrob Agents Chemother. 1980 Nov;18(5):798-806. doi: 10.1128/AAC.18.5.798.
8
Energetics of plasmid-mediated arsenate resistance in Escherichia coli.大肠杆菌中质粒介导的砷酸盐抗性的能量学
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6119-22. doi: 10.1073/pnas.79.20.6119.
9
Organization of unc gene cluster of Escherichia coli coding for proton-translocating ATPase of oxidative phosphorylation.编码氧化磷酸化质子转运ATP酶的大肠杆菌unc基因簇的组织。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7005-9. doi: 10.1073/pnas.77.12.7005.
10
Translocation of phospholipids from the inner to the outer membrane of Escherichia coli.磷脂从大肠杆菌内膜向外膜的转运
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1867-71. doi: 10.1073/pnas.77.4.1867.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.大肠杆菌中L-α-甘油磷酸的主动运输
J Biol Chem. 1964 Sep;239:3098-105.
3
THE ROLE OF PERMEASE IN TRANSPORT.通透酶在运输中的作用。
Biochim Biophys Acta. 1964 Jan 27;79:177-200. doi: 10.1016/0926-6577(64)90050-6.
4
The role of energy coupling in the transport of beta-galactosides by Escherichia coli.能量偶联在大肠杆菌转运β-半乳糖苷中的作用。
J Biol Chem. 1966 May 25;241(10):2200-11.
5
Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate.关于乙二胺四乙酸对大肠菌产生的通透性变化的研究。
J Biol Chem. 1968 May 10;243(9):2373-80.
6
Transport across isolated bacterial cytoplasmic membranes.跨分离的细菌细胞质膜转运。
Biochim Biophys Acta. 1972 Aug 4;265(3):367-416. doi: 10.1016/0304-4157(72)90014-7.
7
The membrane ATPase of Escherichia coli. I. Release into solution, allotopic properties and reconstitution of membrane-bound ATPase.大肠杆菌的膜ATP酶。I. 释放到溶液中、异位性质及膜结合ATP酶的重组。
Biochim Biophys Acta. 1973 May 30;305(2):249-59. doi: 10.1016/0005-2728(73)90173-4.
8
Basic amino acid transport in Escherichia coli. II. Purification and properties of an arginine-specific binding protein.大肠杆菌中碱性氨基酸的转运。II. 精氨酸特异性结合蛋白的纯化及性质
J Biol Chem. 1973 Feb 25;248(4):1211-8.
9
Mutants of Salmonella typhimurium and Escherichia coli pleiotropically defective in active transport.鼠伤寒沙门氏菌和大肠杆菌在主动运输方面多效性缺陷的突变体。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3336-40. doi: 10.1073/pnas.69.11.3336.
10
Coupling of energy to active transport of amino acids in Escherichia coli.能量与大肠杆菌中氨基酸主动运输的偶联
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2663-7. doi: 10.1073/pnas.69.9.2663.
Zotero 插件