大鼠角膜中转运三磷酸腺苷酶的定位
Localization of transport adenosine triphosphatase in rat cornea.
作者信息
Leuenberger P M, Novikoff A B
出版信息
J Cell Biol. 1974 Mar;60(3):721-31. doi: 10.1083/jcb.60.3.721.
Abstract
Increasingly, physiological and biochemical data on the mammalian cornea have emphasized the importance of the endothelial rather than the epithelial layer in regulating the state of hydration of the cornea, via a transport ATPase. This view is supported by the observations reported here. With the Ernst method, the first cytochemical procedure to be readily responsive to ions and ouabain, the major sites of enzyme reaction product are the intercellular spaces of the endothelial layer. This localization was not found by us with the so-called ATPase method of Wachstein and Meisel.
摘要
越来越多关于哺乳动物角膜的生理和生化数据强调，在内皮而非上皮层通过一种转运ATP酶调节角膜水合状态方面的重要性。本文报道的观察结果支持了这一观点。采用恩斯特方法（第一种对离子和哇巴因反应灵敏的细胞化学方法），酶反应产物的主要部位是内皮细胞层的细胞间隙。而我们采用瓦施泰因和迈泽尔所谓的ATP酶方法却未发现这种定位。
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