Wyman L, Goering R V, Novick R P
Genetics. 1974 Apr;76(4):681-702. doi: 10.1093/genetics/76.4.681.
Recombination-deficient mutants of Staphylococcus aureus have been isolated and found to have properties similar to those of recombination-deficient Escherichia coli. In addition, one Rec(-) mutant was found to be defective in the restriction and modification of DNA. There is a marked reduction ( approximately 10(4)-fold) in recombination between penicillinase plasmids in the Rec(-) mutants suggesting that these elements do not encode an efficient recombination system. There is, however, a demonstrable residuum of interplasmid recombination; evidence is lacking on whether this residuum is a plasmid or host function. In the absence of the generalized host recombination system it has been possible to demonstrate that interplasmid recombination occurs during vegetative bacteriophage growth and is presumably mediated by a phage-determined recombination system.
已分离出金黄色葡萄球菌的重组缺陷型突变体,并发现其具有与重组缺陷型大肠杆菌相似的特性。此外,还发现一个Rec(-)突变体在DNA的限制和修饰方面存在缺陷。Rec(-)突变体中青霉素酶质粒之间的重组显著减少(约10^4倍),这表明这些元件不编码有效的重组系统。然而,质粒间重组仍有明显残余;关于这种残余是质粒功能还是宿主功能,尚无证据。在缺乏广义宿主重组系统的情况下,已证实质粒间重组发生在营养性噬菌体生长期间,推测是由噬菌体决定的重组系统介导的。