Garcia M M, Dorward W J, Alexander D C, Magwood S E, McKay K A
Can J Comp Med. 1974 Jul;38(3):222-6.
A preliminary field experiment was undertaken to evaluate the efficacy of alum precipitated toxoids of Sphaerophorus necrophorus prepared from sonicated whole cells and cell fractions to reduce the incidence of bovine abscesses. A total of 108 calves were divided into five groups and treated as follows: I. uninoculated control, II. adjuvant inoculated control, III. 15.5 mg protein of sonicated (fragmented cells) toxoid, IV. 10.5 mg protein of cytoplasmic toxoid. V. 15.5 mg protein of cytoplasmic toxoid. All animals were maintained under similar conditions to those prevailing in feedlots in Alberta. Livers were examined at slaughter. The most promising result was achieved with the injection of 15.5 mg protein of cytoplasmic toxoid. In this treatment group, no scars (healed lesions) were found in the liver and the incidence of liver abscesses was reduced to 10% from the average 35% liver abscesses and scars found in the uninoculated and adjuvant inoculated groups. The toxoid from sonicated whole cells did not reduce liver abscess incidence. These data suggest that the incidence of liver abscesses in cattle fattened in feedlots may be reduced by immunization.
进行了一项初步田间试验，以评估由超声破碎全细胞和细胞组分制备的坏死梭杆菌明矾沉淀类毒素降低牛脓肿发病率的效果。总共108头小牛被分为五组并进行如下处理：I. 未接种对照组，II. 接种佐剂对照组，III. 15.5毫克蛋白质的超声破碎（破碎细胞）类毒素，IV. 10.5毫克蛋白质的细胞质类毒素，V. 15.5毫克蛋白质的细胞质类毒素。所有动物均在与艾伯塔省饲养场相同的条件下饲养。在屠宰时检查肝脏。注射15.5毫克蛋白质的细胞质类毒素取得了最有希望的结果。在该治疗组中，肝脏未发现疤痕（愈合病变），肝脓肿发病率从未接种组和接种佐剂组平均35%的肝脓肿和疤痕降至10%。超声破碎全细胞制备的类毒素未降低肝脓肿发病率。这些数据表明，饲养场育肥牛的肝脓肿发病率可通过免疫接种降低。
