Garcia M M, McKay K A
Can J Comp Med. 1978 Jan;42(1):121-7.
Experiments employing recently developed mouse models indicated that intraperitoneal immunization with the cytoplasm (intracellular fraction) of Fusobacterium necrophorum protected the animals from a lethal challenge of the pathogen. The critical immunization schedule needed to achieve complete protection involved six weekly intraperitoneal doses of the intracellular antigen. Livers of immunized mice were cleared of infecting fusobacterial within 24 hours whereas those of nonimmunized mice harboured increasing numbers of hte bacteria. Sera from both groups did not protect recipient mice form developing liver abscesses after challenge. Sheep immunized intraperitoneally with 20 mg of cytoplasmic protein given in three doseases were protected against the development of abscesses induced by F. necrophorum.
采用最近开发的小鼠模型进行的实验表明，用坏死梭杆菌的细胞质（细胞内组分）进行腹腔免疫可保护动物免受该病原体的致命攻击。实现完全保护所需的关键免疫方案包括每周腹腔注射六次细胞内抗原。免疫小鼠的肝脏在24小时内清除了感染的梭杆菌，而非免疫小鼠的肝脏中细菌数量不断增加。两组小鼠的血清在受到攻击后均不能保护受体小鼠免于形成肝脓肿。用20毫克细胞质蛋白分三次腹腔注射免疫的绵羊可免受坏死梭杆菌诱导的脓肿形成。
