下丘脑组织蛋白酶D：测定及同工酶组成。
Hypothalamic cathepsin D: assay and isoenzyme composition.
作者信息
Akopyan T N, Barchudaryan N A, Karabashyan L V, Arutunyan A A, Lajtha A, Galoyan A A
出版信息
J Neurosci Res. 1979;4(5-6):365-70. doi: 10.1002/jnr.490040505.
PMID:42810
Abstract
A sensitive and convenient method of endopeptidase assay using as substrate globin modified with pyridoxal-5-phosphate was used for determination of acid proteinases in bovine hypothalamus separated by isoelectric focusing. The soluble protein fraction of hypothalamus upon elution from Sephadex gave five peaks of proteinase activity at pH 3.2. The properties indicate that these peaks of endopeptidase activity are isoenzyme forms of cathepsin D.
摘要
一种灵敏且便捷的内肽酶测定方法被用于测定经等电聚焦分离的牛下丘脑酸性蛋白酶，该方法以用磷酸吡哆醛修饰的球蛋白作为底物。从葡聚糖凝胶洗脱后，下丘脑的可溶性蛋白部分在pH 3.2时出现了五个蛋白酶活性峰。这些特性表明，这些内肽酶活性峰是组织蛋白酶D的同工酶形式。
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