Day A J, Fidge N H, Wilkinson G N
J Lipid Res. 1966 Jan;7(1):132-40.
Macrophages obtained from the peritoneal cavity of rabbits were incubated with phosphate-(32)P in order to investigate the synthesis of phospholipid by these cells. After 6 hr of incubation 0.25% of the phosphate added to the medium had been incorporated into phospholipid by the macrophages, mainly into lecithin and sphingomyelin, but partly also into phosphatidyl ethanolamine and inositol phosphatide. The addition of cholesterol to the macrophage suspensions increased the rate of incorporation by 20% with 1 mg of cholesterol added, and 44% with 2.5 mg added. The increase was similar for all the phospholipid fractions, and was not accompanied by any increase in oxygen uptake by the cells. The addition of carbon particles (as a specific check for phagocytic effects) had only a small effect on the rate of incorporation. The data provide support for the concept that cholesterol stimulates phospholipid synthesis by similar cells in the arterial wall during atherogenesis.
从兔腹腔获取的巨噬细胞与磷酸 -（32）P 一起孵育，以研究这些细胞中磷脂的合成。孵育 6 小时后，添加到培养基中的 0.25% 的磷酸盐已被巨噬细胞掺入磷脂中，主要掺入卵磷脂和鞘磷脂，但也有部分掺入磷脂酰乙醇胺和肌醇磷脂。向巨噬细胞悬液中添加胆固醇，添加 1mg 胆固醇时掺入率增加 20%，添加 2.5mg 时增加 44%。所有磷脂组分的增加情况相似，并且细胞的氧摄取没有任何增加。添加碳颗粒（作为吞噬作用的特异性对照）对掺入率只有很小的影响。这些数据为胆固醇在动脉粥样硬化形成过程中刺激动脉壁中类似细胞的磷脂合成这一概念提供了支持。
