Albach R A, Shaffer J G, Watson R H
J Bacteriol. 1965 Oct;90(4):1045-53. doi: 10.1128/jb.90.4.1045-1053.1965.
Albach, Richard A. (Lutheran General Hospital, Park Ridge, Ill.), James G. Shaffer, and Robert H. Watson. Morphology, antigenicity, and nucleic acid content of the Bacteroides sp. used in the culture of Entamoeba histolytica. J. Bacteriol. 90:1045-1053. 1965.-Certain changes in morphology, antigenicity, and nucleic acid content that occur in a culture of Bacteroides sp. in the presence of penicillin G in CLG medium are described. This "variant" is one of seven recovered in several laboratories, all of which are descendants of the original Bacteroides isolated by Shaffer and Frye. Penicillin-inhibited cells of this culture are currently being used in the routine propagation of Entamoeba histolytica in CLG medium. Evidence is presented for the loss of ability to react with antibody in these penicillin-inhibited bacteria in CLG medium, when studied by fluorescent-antibody techniques. The implications of the antigenic changes observed as they pertain to similar antigenic studies of the amoebas are discussed. A pronounced reduction in the ribonucleic acid (RNA) content of such penicillin-inhibited cells was also observed. The potential importance of the changes that occur in the RNA of these cells with respect to considerations of the growth requirements of the amoebas is also discussed.
阿尔巴赫，理查德·A.（伊利诺伊州帕克里奇市路德总医院），詹姆斯·G.·谢弗，以及罗伯特·H.·沃森。用于溶组织内阿米巴培养的拟杆菌属的形态学、抗原性及核酸含量。《细菌学杂志》90:1045 - 1053。1965年。——描述了在CLG培养基中，青霉素G存在时拟杆菌属培养物中发生的形态学、抗原性及核酸含量的某些变化。这种“变体”是在几个实验室中分离得到的七个变体之一，它们都是谢弗和弗莱最初分离的拟杆菌的后代。这种培养物中被青霉素抑制的细胞目前正用于在CLG培养基中常规培养溶组织内阿米巴。当通过荧光抗体技术研究时，有证据表明在CLG培养基中这些被青霉素抑制的细菌与抗体反应的能力丧失。讨论了所观察到的抗原性变化与对阿米巴类似抗原性研究的相关性。还观察到这种被青霉素抑制的细胞中核糖核酸（RNA）含量显著降低。也讨论了这些细胞RNA中发生的变化对于考虑阿米巴生长需求的潜在重要性。
