由脊髓灰质炎病毒I和埃可病毒7的粗制及纯化抗原引发的抗体水平及特异性
Level and specificity of antibodies evoked by crude and purified antigens of poliovirus I and echovirus 7.
作者信息
Frommhagen L H
出版信息
Appl Microbiol. 1965 Nov;13(6):895-8. doi: 10.1128/am.13.6.895-898.1965.
Abstract
Preparations of poliovirus I and echovirus 7, purified by density gradient centrifugation, liquid-phase partition, and anion exchange (diethylaminoethyl) chromatography, have been shown to evoke high antibody levels of substantial specificity in the complement-fixation assay. Certain practical aspects of the three purification methods are discussed. These results argue for the use of purified viral antigens, particularly in view of the simplicity of the purification methods now available.
摘要
通过密度梯度离心、液相分配和阴离子交换（二乙氨基乙基）色谱法纯化的脊髓灰质炎病毒I型和埃可病毒7型制剂，已证明在补体结合试验中能引发具有高度特异性的高抗体水平。讨论了这三种纯化方法的某些实际方面。这些结果支持使用纯化的病毒抗原，特别是考虑到目前可用纯化方法的简便性。
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Level and specificity of antibodies evoked by crude and purified antigens of poliovirus I and echovirus 7.由脊髓灰质炎病毒I和埃可病毒7的粗制及纯化抗原引发的抗体水平及特异性
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本文引用的文献
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A simplified colorimetric test for poliomyelitis virus and antibody.一种用于检测脊髓灰质炎病毒和抗体的简化比色试验。
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