关于马铃薯纺锤块茎类病毒单一感染性种的证据。

Evidence for a single infectious species of potato spindle tuber viroid.

作者信息

Morris T J

出版信息

Intervirology. 1979;11(2):89-96. doi: 10.1159/000149018.

Abstract

Potato spindle tuber viroid (PSTV) has been separated into two electrophoretic species on polyacrylamide gels containing 8 M urea at 60 degrees. Only the slower-migrating electrophoretic form was infectious and is presumably the circular form of the molecule. Both forms had similar base compositions with a 55-58% GC content, similar to the citrus exocortis viroid. Labeling studies in vivo suggest that the viroid is not methylated.

摘要

马铃薯纺锤块茎类病毒（PSTV）在含8M尿素的聚丙烯酰胺凝胶上于60℃条件下可分离为两种电泳条带。只有迁移较慢的电泳形式具有感染性，推测它是该分子的环状形式。两种形式的碱基组成相似，GC含量为55 - 58%，与柑橘裂皮类病毒相似。体内标记研究表明该类病毒未被甲基化。