Lastra R, Acosta J M
Intervirology. 1979;11(4):215-20. doi: 10.1159/000149036.
Leaf extracts containing maize mosaic virus (MMV) were purified by treatment with activated charcoal followed by filtration through Celite. Virus was concentrated from the filtrate by sedimentation onto a sucrose pad and then further purified by centrifugation in a 20--50% continuous sucrose density gradient. Buoyant density of virus particles in sucrose solution was 1.181 g/ml. Nucleic acid extracted from virus preparations reacted with orcinol and behaved like RNA in Pederson's diphenylamine test. It was digested by pancreatic RNase and apparently is single-stranded RNA. Virus preparations also contained lipids. The morphology and constituents of MMV particles are therefore those expected for a rhabdovirus.
含有玉米花叶病毒（MMV）的叶片提取物先用活性炭处理，然后通过硅藻土过滤进行纯化。病毒通过沉降到蔗糖垫层上从滤液中浓缩，然后在20%-50%的连续蔗糖密度梯度中进一步离心纯化。病毒颗粒在蔗糖溶液中的浮力密度为1.181 g/ml。从病毒制剂中提取的核酸与苔黑酚反应，在佩德森二苯胺试验中表现得像RNA。它被胰核糖核酸酶消化，显然是单链RNA。病毒制剂中也含有脂质。因此，MMV颗粒的形态和成分是弹状病毒所预期的。
