Cusack N J, Hickman M E, Born G V
Proc R Soc Lond B Biol Sci. 1979 Nov 30;206(1163):139-44. doi: 10.1098/rspb.1979.0097.
Aggregation of human platelets by ADP and the inhibition of this effect by adenosine are apparently mediated by different receptors. One of the criteria for receptors is that they show stereospecificity for their ligands. We have synthesized L-enantiomers of D-adenosine, AMP and ADP, together with their corresponding photolysable 2-azido analogues so that platelet receptors could be tested for stereospecificity. All of the L-enantiomers were completely inactive as aggregators or inhibitors of platelet function. None of the L-enantiomers changed levels of platelet cAMP. 2-Azido-L-adenosine, AMP and ADP are proposed as useful controls in photoaffinity experiments for non-specific labelling.
ADP引起的人血小板聚集以及腺苷对这种效应的抑制显然是由不同受体介导的。受体的标准之一是它们对其配体表现出立体特异性。我们合成了D-腺苷、AMP和ADP的L-对映体，以及它们相应的可光裂解的2-叠氮类似物，以便测试血小板受体的立体特异性。所有L-对映体作为血小板功能的聚集剂或抑制剂均完全无活性。没有一种L-对映体改变血小板cAMP水平。2-叠氮-L-腺苷、AMP和ADP被提议作为光亲和实验中非特异性标记的有用对照。
