Nomura T, Nakayama N, Murata T, Akazawa T
Plant Physiol. 1967 Mar;42(3):327-32. doi: 10.1104/pp.42.3.327.
The enzymic synthesis of ADP-glucose and UDP-glucose by chloroplastic pyrophosphorylase of bean and rice leaves has been demonstrated by paper chromatographic techniques. In both tissues, the activity of UDP-glucose-pyrophosphorylase was much higher than ADP-glucose-pyrophosphorylase. Glycerate-3-phosphate, phosphoenolpyruvate and fructose-1,6-diphosphate did not stimulate ADP-glucose formation by a pyrophosphorylation reaction. The major metabolic pathway for UDP-glucose utilization appears to be the synthesis of either sucrose or sucrose-P. On the other hand, a specific precursor role of ADP-glucose for synthesizing chloroplast starch by the ADP-glucose-starch transglucosylase reaction is supported by the coupled enzyme system of ADP-glucose-pyrophosphorylase and transglucosylase, isolated from chloroplasts. None of the glycolytic intermediates stimulated the glucose transfer in the enzyme sequence of reaction system employed.
通过纸色谱技术已证明，菜豆和水稻叶片的叶绿体焦磷酸化酶可进行酶促合成ADP-葡萄糖和UDP-葡萄糖。在这两种组织中，UDP-葡萄糖焦磷酸化酶的活性远高于ADP-葡萄糖焦磷酸化酶。3-磷酸甘油酸、磷酸烯醇丙酮酸和1,6-二磷酸果糖不会通过焦磷酸化反应刺激ADP-葡萄糖的形成。UDP-葡萄糖利用的主要代谢途径似乎是蔗糖或磷酸蔗糖的合成。另一方面，从叶绿体中分离出的ADP-葡萄糖焦磷酸化酶和转葡萄糖基酶的偶联酶系统支持了ADP-葡萄糖在通过ADP-葡萄糖-淀粉转葡萄糖基酶反应合成叶绿体淀粉方面的特定前体作用。在所采用的反应系统的酶序列中，没有一种糖酵解中间体能刺激葡萄糖转移。
