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肝脏提取物中乙酰辅酶A测定的内标法。

Internal standards in the estimation of acetyl-CoA in liver extracts.

作者信息

Herrera E, Freinkel N

出版信息

J Lipid Res. 1967 Sep;8(5):515-8.

PMID:4292862
Abstract

The necessity for adding an internal standard to liver extracts during fluorimetric estimation of acetyl-CoA by the malic dehydrogenase-citrate synthase reaction is demonstrated. Addition of acetyl-CoA completely compensates for the inhibitory action of some tissue components. Values for hepatic acetyl-CoA in fed and fasted rats are given.

摘要

通过苹果酸脱氢酶 - 柠檬酸合酶反应荧光法测定肝脏提取物中乙酰辅酶A时添加内标的必要性得到了证明。添加乙酰辅酶A完全补偿了某些组织成分的抑制作用。给出了喂食和禁食大鼠肝脏中乙酰辅酶A的值。

相似文献

1
Internal standards in the estimation of acetyl-CoA in liver extracts.肝脏提取物中乙酰辅酶A测定的内标法。
J Lipid Res. 1967 Sep;8(5):515-8.
2
Determination of coenzyme A and acetyl CoA in tissue extracts.组织提取物中辅酶A和乙酰辅酶A的测定。
Anal Biochem. 1969 May;29(2):293-9. doi: 10.1016/0003-2697(69)90312-1.
3
[On the optical determination of citrate synthase and acetyl-coenzyme A].[关于柠檬酸合酶和乙酰辅酶A的光学测定]
Biochem Z. 1965 Nov 5;343(1):29-43.
4
Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase.乙酰辅酶A的测定。苹果酸脱氢酶中一种污染物的干扰。
J Lipid Res. 1972 Jul;13(4):552-4.
5
Distribution of metabolites between the cytosolic and mitochondrial compartments of hepatocytes isolated from fed rats.从喂食大鼠分离出的肝细胞的胞质溶胶和线粒体区室之间代谢物的分布。
Hoppe Seylers Z Physiol Chem. 1978 Jul;359(7):785-98. doi: 10.1515/bchm2.1978.359.2.785.
6
Citrate synthase interaction with polyphosphate derivatives.柠檬酸合酶与多磷酸盐衍生物的相互作用。
Biochim Biophys Acta. 1967 May 16;139(1):195-8. doi: 10.1016/0005-2744(67)90133-7.
7
DETERMINATION OF ACETYLCOENZYME A IN BRAIN.脑中乙酰辅酶A的测定
J Neurochem. 1965 May;12:451-4. doi: 10.1111/j.1471-4159.1965.tb04245.x.
8
Control of the tricarboxylate cycle and its interactions with glycolysis during acetate utilization in rat heart.大鼠心脏利用乙酸盐过程中三羧酸循环的调控及其与糖酵解的相互作用。
Biochem J. 1970 May;117(4):677-95. doi: 10.1042/bj1170677.
9
ENZYMIC CLEAVAGE OF MALYTL-COENZYME A INTO ACETYL-COENZYME A AND GLYOXYLIC ACID.苹果酰辅酶A酶促裂解为乙酰辅酶A和乙醛酸。
Biochim Biophys Acta. 1965 Jan;96:148-53. doi: 10.1016/0005-2787(65)90618-0.
10
[Citrate synthase, acetyl coenzyme A and citrate in the liver of mice following total-body irradiation].[全身照射后小鼠肝脏中的柠檬酸合酶、乙酰辅酶A和柠檬酸]
Strahlentherapie. 1969 Jan;137(1):88-91.

引用本文的文献

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Multi-Method Quantification of Acetyl-Coenzyme A and Further Acyl-Coenzyme A Species in Normal and Ischemic Rat Liver.正常和缺血大鼠肝中乙酰辅酶 A 及其他酰基辅酶 A 种类的多方法定量。
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Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.量化线粒体乙酰化动力学突出了代谢调节的主要位点。
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On the inability of ketone bodies to serve as the only energy providing substrate for rat heart at physiological work load.
关于在生理工作负荷下酮体无法作为大鼠心脏唯一的能量供应底物的情况。
Basic Res Cardiol. 1983 Jul-Aug;78(4):435-50. doi: 10.1007/BF02070167.
4
Carbohydrate metabolism in pregnancy. VI. Plasma fuels, insulin, liver composition, gluconeogenesis, and nitrogen metabolism during late gestation in the fed and fasted rat.孕期碳水化合物代谢。VI. 进食及禁食大鼠妊娠晚期的血浆燃料、胰岛素、肝脏组成、糖异生及氮代谢
J Clin Invest. 1969 Dec;48(12):2260-72. doi: 10.1172/JCI106192.
5
Effects of hypo- and hyper-thyroidism on liver composition, blood glucose, ketone bodies and insulin in the male rat.甲状腺功能减退和亢进对雄性大鼠肝脏成分、血糖、酮体及胰岛素的影响。
Biochem J. 1972 Jul;128(3):597-604. doi: 10.1042/bj1280597.
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Vitamin K antagonism of coumarin anticoagulation. A dehydrogenase pathway in rat liver is responsible for the antagonistic effect.香豆素抗凝作用的维生素K拮抗。大鼠肝脏中的一条脱氢酶途径负责这种拮抗作用。
Biochem J. 1986 Jun 15;236(3):685-93. doi: 10.1042/bj2360685.