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An improved method for the purification of staphylococcal nuclease.

作者信息

Fuchs S, Cuatrecasas P, Anfinsen C B

出版信息

J Biol Chem. 1967 Oct 25;242(20):4768-70.

PMID:4294026
Abstract
摘要

相似文献

1
An improved method for the purification of staphylococcal nuclease.一种改进的葡萄球菌核酸酶纯化方法。
J Biol Chem. 1967 Oct 25;242(20):4768-70.
2
The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. II. The amino acid sequences of tryptic and chymotryptic peptides.金黄色葡萄球菌一种细胞外核酸酶的氨基酸序列。II. 胰蛋白酶和胰凝乳蛋白酶肽段的氨基酸序列。
J Biol Chem. 1967 Oct 25;242(20):4736-51.
3
Susceptibility of dinucleotides bearing either 3'- or 5'-monophosphate to micrococcal nuclease.携带3'-或5'-单磷酸的二核苷酸对微球菌核酸酶的敏感性。
J Biol Chem. 1969 Dec 25;244(24):6559-65.
4
Structure of bis(methylguanidinium) monohydrogen orthophosphate. A model for the arginine-phosphate interactions at the active site of staphylococcal nuclease and other phosphohydrolytic enzymes.
J Am Chem Soc. 1974 Jul 10;96(14):4471-8. doi: 10.1021/ja00821a020.
5
The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. 3. Complete amino acid sequence.金黄色葡萄球菌一种细胞外核酸酶的氨基酸序列。3. 完整氨基酸序列。
J Biol Chem. 1967 Oct 25;242(20):4752-8.
6
An experimental approach to the study of the folding of staphylococcal nuclease.一种研究葡萄球菌核酸酶折叠的实验方法。
J Biol Chem. 1969 Jul 25;244(14):3864-75.
7
The interaction of nucleotides with the active site of staphylococcal nuclease. Spectrophotometric studies.核苷酸与葡萄球菌核酸酶活性位点的相互作用。分光光度法研究。
J Biol Chem. 1967 Oct 25;242(20):4759-67.
8
The action of staphylococcal nuclease on synthetic substrates.
Biochemistry. 1969 Jun;8(6):2277-84. doi: 10.1021/bi00834a007.
9
Studies on extracellular proteins from Staphylococcus aureus. VI. Production and purification of -haemolysin in large scale.金黄色葡萄球菌细胞外蛋白质的研究。VI.大规模生产和纯化α-溶血素。
Biochim Biophys Acta. 1971 Jul 21;242(1):288-307. doi: 10.1016/0005-2744(71)90109-4.
10
Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus.金黄色葡萄球菌细胞外核酸酶的催化特性及特异性
J Biol Chem. 1967 Apr 10;242(7):1541-7.

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Nuclease modulates biofilm formation in community-associated methicillin-resistant Staphylococcus aureus.核酸酶调节社区获得性耐甲氧西林金黄色葡萄球菌生物膜的形成。
PLoS One. 2011;6(11):e26714. doi: 10.1371/journal.pone.0026714. Epub 2011 Nov 11.
2
The pK(a) values of acidic and basic residues buried at the same internal location in a protein are governed by different factors.埋藏在蛋白质同一内部位置的酸性和碱性残基的pK(a)值受不同因素支配。
J Mol Biol. 2009 May 29;389(1):34-47. doi: 10.1016/j.jmb.2009.03.039. Epub 2009 Mar 24.
3
A buried lysine that titrates with a normal pKa: role of conformational flexibility at the protein-water interface as a determinant of pKa values.
一个具有正常pKa值的埋藏赖氨酸:蛋白质-水界面处的构象灵活性作为pKa值决定因素的作用。
Protein Sci. 2008 May;17(5):833-45. doi: 10.1110/ps.073397708. Epub 2008 Mar 27.
4
Reorientational dynamics of enzymes adsorbed on quartz: a temperature-dependent time-resolved TIRF anisotropy study.吸附在石英上的酶的重取向动力学:一项温度依赖性时间分辨全内反射荧光各向异性研究。
Biophys J. 2003 Apr;84(4):2533-41. doi: 10.1016/S0006-3495(03)75058-9.
5
Least activation path for protein folding: investigation of staphylococcal nuclease folding by stopped-flow circular dichroism.蛋白质折叠的最小激活路径:通过停流圆二色性研究葡萄球菌核酸酶的折叠
Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2539-44. doi: 10.1073/pnas.93.6.2539.
6
Thermodynamic characterization of an equilibrium folding intermediate of staphylococcal nuclease.葡萄球菌核酸酶平衡折叠中间体的热力学表征
Protein Sci. 1994 Dec;3(12):2175-84. doi: 10.1002/pro.5560031203.
7
Salt induced transitions of chromatin core particles studied by tyrosine fluorescence anisotropy.通过酪氨酸荧光各向异性研究盐诱导的染色质核心颗粒转变。
Nucleic Acids Res. 1980 Aug 25;8(16):3517-34. doi: 10.1093/nar/8.16.3517.
8
Histone hyperacetylation has little effect on the higher order folding of chromatin.
Nucleic Acids Res. 1983 Jun 25;11(12):4065-75. doi: 10.1093/nar/11.12.4065.
9
The high mobility group proteins HMG 14 and 17, do not prevent the formation of chromatin higher order structure.高迁移率族蛋白HMG 14和HMG 17并不阻止染色质高级结构的形成。
Nucleic Acids Res. 1982 Mar 25;10(6):2007-16. doi: 10.1093/nar/10.6.2007.
10
Nuclear magnetic resonance studies of the structure and binding sites of enzymes. X. Preparation of selectively deuterated analogs of staphylococcal nuclease.酶的结构与结合位点的核磁共振研究。X. 葡萄球菌核酸酶选择性氘代类似物的制备。
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1396-403. doi: 10.1073/pnas.64.4.1396.