Gilliland S E, Speck M L
Appl Microbiol. 1967 Sep;15(5):1038-44. doi: 10.1128/am.15.5.1038-1044.1967.
Electrohydraulic shock was shown to produce oxidation reactions which inactivated certain compounds important in cellular metabolism. Enzymes that were inactivated included lactic dehydrogenase, trypsin, and proteinases of Bacillus subtilis. Free sulfhydryl groups and reduced nicotinamide adenine dinucleotide were oxidized. Adenosine triphosphate was destroyed, but deoxyribonucleic acid was not affected. Intracellular material of Escherichia coli lost its ability to absorb at 260 mmu after electrohydraulic shock. The bactericidal mechanism involved appeared to be due to nonselective oxidation reactions produced by high-voltage discharges in water. These oxidation reactions were probably mediated by free radicals produced in the water.
电液压冲击被证明会产生氧化反应，这些反应会使细胞代谢中某些重要的化合物失活。失活的酶包括乳酸脱氢酶、胰蛋白酶和枯草芽孢杆菌的蛋白酶。游离巯基和还原型烟酰胺腺嘌呤二核苷酸被氧化。三磷酸腺苷被破坏，但脱氧核糖核酸未受影响。电液压冲击后，大肠杆菌的细胞内物质失去了在260毫微米处吸收的能力。所涉及的杀菌机制似乎是由于水中高压放电产生的非选择性氧化反应。这些氧化反应可能是由水中产生的自由基介导的。
