Satyaswaroop P G, Bressler R S, de la Pena M M, Gurpide E
J Clin Endocrinol Metab. 1979 Apr;48(4):639-41. doi: 10.1210/jcem-48-4-639.
A simple method for isolation of glands from human endometrium has been developed. The procedure involves collagenase digestion of the endometrial tissue and filtration through sieves of various pore sizes. Isolated glands retained on the sieves were washed and collected in culture dishes. Tubular organization of the isolated glands was ascertained by examination of the preparations under inverted microscope and light microscopy of stained sections. The appearance of the glands was found to reflect different functional states of the endometrium and, possibly, to reveal abnormalities. Growth of monolayers of epithelial cells derived from the glands was observed within 24 h of culturing. Electron microscopy of the cells in 7-day monolayer preparations from both proliferative and secretory endometrium revealed the characteristic features of human endometrial epithelial cells, viz. presence of microvilli and desmosome-like junctions. Nuclear bodies were observed in cells derived from both types of endometrium.
已开发出一种从人子宫内膜分离腺体的简单方法。该程序包括用胶原酶消化子宫内膜组织，并通过不同孔径的筛网进行过滤。保留在筛网上的分离腺体经冲洗后收集到培养皿中。通过在倒置显微镜下检查制剂以及对染色切片进行光学显微镜检查来确定分离腺体的管状结构。发现腺体的外观反映了子宫内膜的不同功能状态，并且可能揭示异常情况。培养24小时内观察到源自腺体的上皮细胞单层生长。对增殖期和分泌期子宫内膜7天单层制剂中的细胞进行电子显微镜检查，揭示了人子宫内膜上皮细胞的特征，即存在微绒毛和类桥粒连接。在两种类型子宫内膜来源的细胞中均观察到核体。
