通过超速离心法分离香草酸O-脱甲基酶和原儿茶酸3,4-加氧酶。
The separation of vanillate O-demethylase from protocatechuate 3,4-oxygenase by ultracentrifugation.
作者信息
Cartwright N J, Buswell J A
出版信息
Biochem J. 1967 Nov;105(2):767-70. doi: 10.1042/bj1050767.
Abstract
- Protocatechuate 3,4-oxygenase in the soluble part of a cell-free extract of Pseudomonas fluorescens (strain T) sedimented more rapidly than vanillate O-demethylase under specified conditions in a preparative ultracentrifuge. 2. The supernatant from this process contained vanillate O-demethylase and formaldehyde dehydrogenase, and when supplemented with NADH oxidized vanillate with an uptake of 1 mole of oxygen/mole of substrate and accumulation of protocatechuate. 3. This uptake was decreased to 0.5mole/mole of substrate in the presence of semicarbazide as trapping agent for formaldehyde. 4. Reasons are presented for the process of methyl group removal from vanillate being oxidative demethylation.
摘要
- 在制备型超速离心机的特定条件下，荧光假单胞菌（菌株T）无细胞提取物可溶部分中的原儿茶酸3,4-加氧酶比香草酸O-脱甲基酶沉降得更快。2. 此过程的上清液含有香草酸O-脱甲基酶和甲醛脱氢酶，当补充NADH时，能氧化香草酸，每摩尔底物摄取1摩尔氧气，并积累原儿茶酸。3. 在存在氨基脲作为甲醛捕获剂的情况下，这种摄取量降至每摩尔底物0.5摩尔。4. 文中给出了香草酸甲基去除过程为氧化脱甲基的原因。
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