Schlaff S, Rosen S W, Roth J
J Clin Invest. 1968 Jul;47(7):1722-9. doi: 10.1172/JCI105862.
Antibodies directed against human follicle-stimulating hormone (FSH) were demonstrated in rabbit serum by neutralization of biological activity. Antibodies that bound FSH-(131)I were produced in rabbits and guinea pigs by repeated injections of FSH. By (131)I immunochemical methods, we found that at least 90% of the FSH-(131)I-binding antibody failed to distinguish the four human glycoprotein hormones: FSH, luteinizing hormone, chorionic gonadotropin, and thyrotropin, purified as well as endogenous hormone in plasma. Neither growth hormone, adrenocorticotropin, nor a variety of glycoproteins or animal plasmas were able to react with these antibodies.
通过生物活性的中和作用，在兔血清中证实了针对人促卵泡激素（FSH）的抗体。通过反复注射FSH，在兔和豚鼠体内产生了能结合FSH-（131）I的抗体。通过（131）I免疫化学方法，我们发现至少90%的FSH-（131）I结合抗体无法区分四种人糖蛋白激素：FSH、促黄体生成素、绒毛膜促性腺激素和促甲状腺激素，无论是纯化的还是血浆中的内源性激素。生长激素、促肾上腺皮质激素，以及多种糖蛋白或动物血浆都不能与这些抗体发生反应。
