Frohman M A, Frohman L A, Goldman M B, Goldman J N
J Lab Clin Med. 1979 Apr;93(4):614-21.
Heat killed, formalinized Cowan I strain Staphylococcus aureus (PASA) was examined for its utility as a solid-phase immunoadsorbent in various RIA procedures. Specific antibody could be adsorbed to PASA and then reacted with radiolabeled and unlabeled hormone, or alternatively, PASA could be added to antibody-hormone mixture in order to separate antibody-bound from free antigen. Both methods compared favorably with the more standard double-antibody method in each system tested. Antibodies produced by rabbits, guinea pigs, monkeys, and humans were precipitated equally by PASA and double antibody, whereas antibody raised in sheep was precipitated much less by PASA than by double antibody. Antibody bound to PASA was found to reach equilibrium of binding more slowly than unbound antibody for both rapid (TRH) and slow (rPRL) reactions. Binding of either free of complexed IgG by PASA, however, was extremely rapid, reaching equilibrium in less than 30 sec. This rapid binding was utilized in kinetic experiments which would not have been possible with slower-reacting precipitating agents. The versatility permitted by this propertly constitutes one of the most useful characteristics of PASA. Other important attributes of PASA include its convenience, economy, and wide applicability.
对热灭活、经福尔马林处理的考恩I株金黄色葡萄球菌（PASA）作为各种放射免疫分析（RIA）程序中的固相免疫吸附剂的效用进行了检测。特异性抗体可吸附到PASA上，然后与放射性标记和未标记的激素反应，或者，可将PASA添加到抗体-激素混合物中，以分离结合抗体的抗原与游离抗原。在每个测试系统中，这两种方法与更标准的双抗体法相比都具有优势。由兔、豚鼠、猴和人产生的抗体被PASA和双抗体沉淀的程度相同，而羊产生的抗体被PASA沉淀的程度远低于被双抗体沉淀的程度。对于快速反应（促甲状腺激素释放激素，TRH）和慢速反应（大鼠催乳素，rPRL），发现与PASA结合的抗体比未结合抗体达到结合平衡的速度更慢。然而，PASA对游离或复合IgG的结合极其迅速，在不到30秒内达到平衡。这种快速结合被用于动力学实验，而使用反应较慢的沉淀剂则无法进行这类实验。这一特性所带来的多功能性是PASA最有用的特性之一。PASA的其他重要特性包括其便利性、经济性和广泛适用性。
