脱硫脱硫弧菌L-丙氨酸脱氢酶的纯化及性质
Purification and properties of L-alanine dehydrogenase from Desulfovibrio desulfuricans.
作者信息
Germano G J, Anderson K E
出版信息
J Bacteriol. 1968 Jul;96(1):55-60. doi: 10.1128/jb.96.1.55-60.1968.
Abstract
The l-alanine dehydrogenase from cell-free extracts of Desulfovibrio desulfuricans was purified approximately 56-fold. The Michaelis constants for the substrates of the amination reaction and the pH optima for the reactions catalyzed by this enzyme closely agree with those reported for other l-alanine dehydrogenases. Pyruvate was found to inhibit the amination reaction. The enzyme was absolutely specific for l-alanine and nicotinamide adenine dinucleotide. Its sensitivity to para-chloromecuribenzoate suggests that sulfhydryl groups may be necessary for enzymatic activity. These extracts also contained a nicotinamide adenine dinucleotide phosphate-specific glutamic dehydrogenase which was separated from the l-alanine dehydrogenase during purification.
摘要
从脱硫脱硫弧菌无细胞提取物中获得的L-丙氨酸脱氢酶被纯化了约56倍。该酶催化的胺化反应底物的米氏常数以及反应的最适pH值与其他L-丙氨酸脱氢酶所报道的数值非常吻合。发现丙酮酸会抑制胺化反应。该酶对L-丙氨酸和烟酰胺腺嘌呤二核苷酸具有绝对特异性。它对对氯汞苯甲酸的敏感性表明，巯基可能是酶活性所必需的。这些提取物还含有一种烟酰胺腺嘌呤二核苷酸磷酸特异性谷氨酸脱氢酶，在纯化过程中它与L-丙氨酸脱氢酶分离。
相似文献
1
Purification and properties of L-alanine dehydrogenase from Desulfovibrio desulfuricans.脱硫脱硫弧菌L-丙氨酸脱氢酶的纯化及性质
J Bacteriol. 1968 Jul;96(1):55-60. doi: 10.1128/jb.96.1.55-60.1968.
2
Partial purification and properties of Cunninghamella elegans L-alanine dehydrogenase.雅致小克银汉霉L-丙氨酸脱氢酶的部分纯化及性质
Zentralbl Mikrobiol. 1989;144(4):231-40.
3
Thermostable alanine dehydrogenase from thermophilic Bacillus sphaericus DSM 462. Purification, characterization and kinetic mechanism.嗜热球形芽孢杆菌DSM 462来源的耐热丙氨酸脱氢酶。纯化、表征及动力学机制。
Eur J Biochem. 1990 Aug 17;191(3):715-20. doi: 10.1111/j.1432-1033.1990.tb19180.x.
4
Purification and properties of alanine dehydrogenase from Bacillus sphaericus.球形芽孢杆菌丙氨酸脱氢酶的纯化及性质
Eur J Biochem. 1979 Oct;100(1):29-30. doi: 10.1111/j.1432-1033.1979.tb02030.x.
5
Alanine dehydrogenase from Enterobacter aerogenes: purification, characterization, and primary structure.产气肠杆菌丙氨酸脱氢酶：纯化、表征及一级结构
Biosci Biotechnol Biochem. 1998 Dec;62(12):2357-63. doi: 10.1271/bbb.62.2357.
6
Alanine dehydrogenase from Streptomyces fradiae. Purification and properties.弗氏链霉菌的丙氨酸脱氢酶。纯化及性质
Eur J Biochem. 1989 Jan 15;179(1):221-7. doi: 10.1111/j.1432-1033.1989.tb14544.x.
7
Alanine dehydrogenase from soybean nodule bacteroids: purification and properties.来自大豆根瘤类菌体的丙氨酸脱氢酶：纯化及性质
Arch Biochem Biophys. 1993 Aug 1;304(2):379-85. doi: 10.1006/abbi.1993.1365.
8
Regulation of alanine dehydrogenase in Bacillus (licheniformis).地衣芽孢杆菌中丙氨酸脱氢酶的调控
J Bacteriol. 1974 May;118(2):590-7. doi: 10.1128/jb.118.2.590-597.1974.
9
THE OXIDATION OF D- AND L-GLYCERATE BY RAT LIVER.大鼠肝脏对D-甘油酸和L-甘油酸的氧化作用
Biochem J. 1965 Feb;94(2):353-67. doi: 10.1042/bj0940353.
10
Production of racemic lactic acid in Pediococcus cerevisiae cultures by two lactate dehydrogenases.两种乳酸脱氢酶在啤酒片球菌培养物中产生消旋乳酸。
J Bacteriol. 1975 Feb;121(2):600-7. doi: 10.1128/jb.121.2.600-607.1975.
引用本文的文献
1
Crystallization and preliminary X-ray analysis of an alanine dehydrogenase from Bacillus megaterium WSH-002.巨大芽孢杆菌WSH-002丙氨酸脱氢酶的结晶及初步X射线分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):934-6. doi: 10.1107/S1744309113019672. Epub 2013 Jul 27.
2
Alanine dehydrogenase of the beta-lactam antibiotic producer Streptomyces clavuligerus.β-内酰胺抗生素产生菌克拉维链霉菌的丙氨酸脱氢酶。
Arch Microbiol. 1980 Mar;125(1-2):137-42. doi: 10.1007/BF00403210.
3
Ammonia assimilation and synthesis of alanine, aspartate, and glutamate in Methanosarcina barkeri and Methanobacterium thermoautotrophicum.巴氏甲烷八叠球菌和嗜热自养甲烷杆菌中的氨同化以及丙氨酸、天冬氨酸和谷氨酸的合成
J Bacteriol. 1982 Jun;150(3):1357-65. doi: 10.1128/jb.150.3.1357-1365.1982.
4
Serine biosynthesis in Desulfovibrio desulfuricans.脱硫脱硫弧菌中的丝氨酸生物合成
J Bacteriol. 1969 Sep;99(3):893-4. doi: 10.1128/jb.99.3.893-894.1969.
5
Ammonia assimilation in blue-green algae.蓝藻中的氨同化作用。
Arch Mikrobiol. 1973;89(1):15-22. doi: 10.1007/BF00409395.
6
Partial purification and properties of Halobacterium cutirubrum L-alanine dehydrogenase.深红嗜盐菌L-丙氨酸脱氢酶的部分纯化及性质
Biochem J. 1977 Feb 1;161(2):313-20. doi: 10.1042/bj1610313.
7
Alanine dehydrogenase of the N2-fixing blue-green alga, Anabaena cylindrica.固氮蓝藻圆柱鱼腥藻的丙氨酸脱氢酶
Arch Microbiol. 1975 Sep;107(2):115-24. doi: 10.1007/BF00446830.
本文引用的文献
1
Alanine synthesis and carbohydrate oxidation by smooth Brucella abortus.光滑型流产布鲁氏菌的丙氨酸合成与碳水化合物氧化
J Bacteriol. 1951 Jul;62(1):97-105. doi: 10.1128/jb.62.1.97-105.1951.
2
Distribution of L-alanine dehydrogenase and L-glutamate dehydrogenase in Bacilli.芽孢杆菌中L-丙氨酸脱氢酶和L-谷氨酸脱氢酶的分布
Biochim Biophys Acta. 1959 Nov;36:288-9. doi: 10.1016/0006-3002(59)90111-8.
3
PURIFICATION AND PROPERTIES OF L-ALANINE DEHYDROGENASE FROM VEGETATIVE CELLS OF BACILLUS CEREUS.蜡样芽孢杆菌营养细胞中L-丙氨酸脱氢酶的纯化及性质
J Bacteriol. 1964 Jan;87(1):68-74. doi: 10.1128/jb.87.1.68-74.1964.
4
Enzyme systems in the mycobacteria. VII. Purification, properties and mechanism of action of the alanine dehydrogenase.分枝杆菌中的酶系统。VII. 丙氨酸脱氢酶的纯化、性质及作用机制
Biochim Biophys Acta. 1959 Aug;34:527-39. doi: 10.1016/0006-3002(59)90305-1.
5
Glutamic dehydrogenase. III. The order of substrate addition in the enzymatic reaction.谷氨酸脱氢酶。III. 酶促反应中底物添加的顺序。
J Biol Chem. 1959 Nov;234:2891-6.
6
Steroid hormone activation of L-alanine oxidation catalyzed by a subunit of crystalline glutamic dehydrogenase.晶体谷氨酸脱氢酶一个亚基催化的L-丙氨酸氧化的类固醇激素激活作用。
Proc Natl Acad Sci U S A. 1961 Mar 15;47(3):270-8. doi: 10.1073/pnas.47.3.270.
7
Intermediate metabolism of aerobic spores. V. The purification and properties of L-alanine dehydrogenase.需氧芽孢杆菌的中间代谢。V. L-丙氨酸脱氢酶的纯化及性质
Arch Biochem Biophys. 1960 Dec;91:290-9. doi: 10.1016/0003-9861(60)90503-8.
8
The substrate specificity of L-alanine dehydrogenase.L-丙氨酸脱氢酶的底物特异性。
Biochim Biophys Acta. 1961 Mar 18;48:47-55. doi: 10.1016/0006-3002(61)90513-3.
9
Studies in amino acid biogenesis: the synthesis of alanine from pyruvate and ammonia.氨基酸生物合成的研究：由丙酮酸和氨合成丙氨酸。
J Gen Microbiol. 1956 Aug;15(1):106-20. doi: 10.1099/00221287-15-1-106.
10
Inhibition of complement activity by di-isopropyl fluorophosphate.二异丙基氟磷酸酯对补体活性的抑制作用。
Nature. 1955 Dec 3;176(4492):1073. doi: 10.1038/1761073a0.
Zotero 插件