Moroff G, Chang C H
Transfusion. 1979 Nov-Dec;19(6):704-18. doi: 10.1046/j.1537-2995.1979.19680104096.x.
The aggregating response of human platelets stored at 22 C for 72 hours has been studied. Platelets were stored as platelet-rich plasma in order to maintain the plasma pH essentially constant. The response to ADP and collagen decreased with time, but the decrease was less with relatively high concentrations of the aggregating agents. The response to the ionophore A23187 was essentially unaltered during the storage period. Synergistic aggregation with combinations of ADP, collagen and ionophore A23187 was observed with platelets stored for 72 hours under conditions where singly each of the stimuli caused little or no effect. Stored platelets underwent reversible aggregation over a wide range of ADP concentrations but irreversible aggregation was observed in the presence of nonaggregating concentrations of collagen or ionophore A23187. The inhibition of aggregation by PGE1 or cyclic AMP was facilitated as a result of storage. It is suggested that the decreased response toward ADP and collagen reflects a reduced ability on the part of the platelets to mobilize calcium.
对在22℃下储存72小时的人血小板的聚集反应进行了研究。血小板以富含血小板血浆的形式储存，以维持血浆pH基本恒定。对ADP和胶原蛋白的反应随时间下降，但在相对高浓度的聚集剂作用下下降较少。在储存期间，对离子载体A23187的反应基本未改变。在单独每种刺激作用很小或没有作用的条件下，观察到储存72小时的血小板与ADP、胶原蛋白和离子载体A23187组合时出现协同聚集。储存的血小板在很宽的ADP浓度范围内发生可逆聚集，但在存在非聚集浓度的胶原蛋白或离子载体A23187时观察到不可逆聚集。储存导致PGE1或环磷酸腺苷对聚集的抑制作用增强。有人提出，对ADP和胶原蛋白反应的降低反映了血小板动员钙的能力下降。
