将碘脱氧尿苷掺入感染猿猴病毒40后合成的细胞脱氧核糖核酸中。
Incorporation of iododeoxyuridine into cellular deoxyribonucleic acid synthesized after infection with simian virus 40.
作者信息
Maass G, Werchau H, Brandner G, Haas R
出版信息
J Virol. 1968 Jul;2(7):723-7. doi: 10.1128/JVI.2.7.723-727.1968.
Primary monkey kidney cells (Cerocpithecus aethiops) in the stationary phase of growth were labeled with (14)C-thymidine for 24 hr prior to infection with simian virus 40 (strain 777). (3)H-deoxyadenosine and 5-iodo-2'-deoxyuridine (IUdR) were added to some of the cultures 24, 48, or 72 hr after infection; 24 hr later the deoxyribonucleic acid (DNA) was extracted from these cultures and centrifuged in a CsCl density gradient. The portion of DNA which had become heavier because of incorporation of IUdR could be seen as a second peak in the sedimentation profile. This peak contained (14)C as well as (3)H activity. The possibility that the (14)C-labeled cellular DNA might be degraded and used for the synthesis of viral DNA could be excluded. On the basis of these results, it must be assumed that the infection of monkey kidney cells with simian virus 40 induces the synthesis of cellular DNA.
在生长静止期的原代猴肾细胞（非洲绿猴肾细胞），在用猿猴病毒40（777株）感染前，用（14）C-胸腺嘧啶核苷标记24小时。在感染后24、48或72小时，向部分培养物中加入（3）H-脱氧腺苷和5-碘-2'-脱氧尿苷（IUdR）；24小时后，从这些培养物中提取脱氧核糖核酸（DNA），并在CsCl密度梯度中进行离心。由于IUdR掺入而变重的那部分DNA在沉降图谱中可表现为第二个峰。这个峰含有（14）C以及（3）H活性。可以排除（14）C标记的细胞DNA可能被降解并用于病毒DNA合成的可能性。基于这些结果，必须假定猿猴病毒40感染猴肾细胞会诱导细胞DNA的合成。
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