Pansu D, Bellaton C, Bronner F
J Nutr. 1979 Mar;109(3):508-12. doi: 10.1093/jn/109.3.508.
Rats were fed a purified diet containing 30% lactose and calcium absorption was measured in duodenal loops in situ following instillation of 1.25 or 10 mM CaCl2 solutions. Lactose feeding caused absorption to be depressed from 88 to 69% (1.25 mM Ca solution) and from 71 to 43% (10 mM Ca solution). The effect of lactose feeding was more pronounced in 5-month old rats than in 2-month old rats. In the lactose-fed rats, calcium-binding protein (CaBP), measured by a competitive binding assay following partial purification, was depressed on the average from 24 to 10 nmoles Ca bound per mg protein. The effect of the lactose ingestion can be likened to the effect expected from continued high calcium intake, i.e., a decrease in the efficiency of calcium absorption and a decrease in CaBP.
给大鼠喂食含30%乳糖的纯化日粮，在十二指肠肠袢原位滴注1.25或10 mM氯化钙溶液后测量钙吸收。喂食乳糖导致钙吸收从88%降至69%（1.25 mM钙溶液），从71%降至43%（10 mM钙溶液）。喂食乳糖的影响在5月龄大鼠中比在2月龄大鼠中更明显。在喂食乳糖的大鼠中，通过部分纯化后的竞争性结合测定法测量的钙结合蛋白（CaBP）平均从每毫克蛋白质结合24纳摩尔钙降至10纳摩尔钙。摄入乳糖的影响可类似于持续高钙摄入预期产生的影响，即钙吸收效率降低和CaBP减少。
