成年和胎儿豚鼠肝脏组织溶酶体的渗透稳定性
The osmotic stability of lysosomes from adult and foetal guinea-pig liver tissue.
作者信息
Turnbull J M, Neil M W
出版信息
Biochem J. 1969 Feb;111(4):503-7. doi: 10.1042/bj1110503.
Abstract
- Lysosome-rich fractions were obtained from foetal liver tissues as early as 35 days uterine age. Foetal lysosomes showed the same ;structure-linked latency' and acid hydrolytic potentiality characteristic of their adult counterparts. 2. The osmotic stability of lysosome-rich fraction from foetal guinea-pig liver tissue was greater than that of the corresponding adult lysosome fractions, p-nitrophenyl-phosphatase being used as marker enzyme. 3. The observation was confirmed by using beta-glycerophosphatase and phenolphthalein beta-glucuronidase as alternative marker enzymes. p-Nitrophenyl phosphate and beta-glycerophosphate appear to act as substrates for the same enzyme. 4. By using p-nitrophenylphosphatase activity measurements it was shown that the osmotic stability of foetal lysosomal fractions decreased with increasing foetal age, but at no time achieved the degree of osmotic instability characteristic of adult lysosomal fractions. 5. The correlation of these findings with the intracellular environment of lysosomes is discussed.
摘要
- 早在子宫龄35天时，就从胎儿肝脏组织中获得了富含溶酶体的组分。胎儿溶酶体显示出与其成年对应物相同的“结构相关潜伏性”和酸性水解潜力特性。2. 以对硝基苯磷酸酶作为标记酶，胎儿豚鼠肝脏组织中富含溶酶体的组分的渗透稳定性高于相应的成年溶酶体组分。3. 通过使用β-甘油磷酸酶和酚酞β-葡萄糖醛酸酶作为替代标记酶，证实了这一观察结果。对硝基苯磷酸酯和β-甘油磷酸似乎作为同一种酶的底物。4. 通过测量对硝基苯磷酸酶活性表明，胎儿溶酶体组分的渗透稳定性随着胎龄增加而降低，但从未达到成年溶酶体组分特有的渗透不稳定性程度。5. 讨论了这些发现与溶酶体细胞内环境的相关性。
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