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Plasma membranes of the rat liver. Isolation and enzymatic characterization of a fraction rich in bile canaliculi.大鼠肝脏的质膜。富含胆小管的部分的分离及酶学特性分析。
J Cell Biol. 1969 Apr;41(1):124-32. doi: 10.1083/jcb.41.1.124.
2
Subfractionation of rat liver plasma membrane. Uneven distribution of plasma membrane-bound enzymes on the liver cell surface.大鼠肝细胞膜的亚分级分离。肝细胞膜结合酶在肝细胞表面的分布不均。
Biochim Biophys Acta. 1975 Nov 17;413(1):52-64. doi: 10.1016/0005-2736(75)90058-9.
3
Properties of (Na+ plus K+)-activated ATPase in rat liver plasma membranes enriched with bile canaliculi.富含胆小管的大鼠肝细胞膜中（钠加钾）激活的ATP酶的特性
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4
Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte.大鼠肝细胞表面膜的功能极性。从肝细胞血窦面、胆小管面及相邻表面分离和鉴定质膜亚组分。
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5
Alteration of bile canalicular enzymes in cholestasis. A possible cause of bile secretory failure.胆汁淤积时胆小管酶的改变。胆汁分泌衰竭的一个可能原因。
J Clin Invest. 1973 Apr;52(4):765-75. doi: 10.1172/JCI107239.
6
5'-nucleotidase of plasma membranes of the rat liver: studies on subcellular distribution.大鼠肝脏质膜的5'-核苷酸酶：亚细胞分布研究
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8
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Isolation and characterization of Golgi membranes from bovine liver.牛肝高尔基体膜的分离与特性分析
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Tissue fraction-past and present.组织分级——过去与现在。
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Uptake, accumulation and release of ouabain by isolated rat hepatocytes.离体大鼠肝细胞对哇巴因的摄取、蓄积和释放
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本文引用的文献

1
A re-examination of the structure of the mammalian liver; the hepatic lobule and its relation to the vascular and biliary systems.对哺乳动物肝脏结构的重新审视；肝小叶及其与血管和胆道系统的关系。
Am J Anat. 1949 Nov;85(3):379-456, 15 pl. doi: 10.1002/aja.1000850303.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Membrane adenosine triphosphatase as a participant in the active transport of sodium and potassium in the human erythrocyte.膜三磷酸腺苷酶作为人类红细胞中钠和钾主动转运的参与者。
J Biol Chem. 1960 Jun;235:1796-802.
4
ENZYMATIC BASIS FOR ACTIVE TRANSPORT OF NA+ AND K+ ACROSS CELL MEMBRANE.钠离子和钾离子跨细胞膜主动运输的酶学基础
Physiol Rev. 1965 Jul;45:596-617. doi: 10.1152/physrev.1965.45.3.596.
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A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.一种用于电子显微镜的简化柠檬酸铅染色法。
J Cell Biol. 1965 May;25(2):407-8. doi: 10.1083/jcb.25.2.407.
6
THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.肝微粒体的一氧化碳结合色素。I. 其血红蛋白性质的证据。
J Biol Chem. 1964 Jul;239:2370-8.
7
STUDIES ON CHOLESTASIS. A RE-EVALUATION OF THE FINE STRUCTURE OF NORMAL HUMAN BILE CANALICULI.胆汁淤积的研究。对正常人胆小管精细结构的重新评估。
Lab Invest. 1964 Aug;13:840-64.
8
STUDIES ON PLASMA MEMBRANES. I. CHEMICAL COMPOSITION AND ENZYME CONTENT OF PLASMA MEMBRANES ISOLATED FROM RAT LIVER.细胞膜研究。I. 从大鼠肝脏分离的细胞膜的化学组成和酶含量
Biochim Biophys Acta. 1964 Jul 15;90:126-45. doi: 10.1016/0304-4165(64)90125-4.
9
COMPARATIVE EFFECTS OF L-HISTIDINE ON THE ACTIVITIES OF 5'-NUCLEOTIDASE AND ALKALINE PHOSPHATASE.L-组氨酸对5'-核苷酸酶和碱性磷酸酶活性的比较作用
J Biol Chem. 1963 Oct;238:3420-7.
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Some structural features of the bile canaliculus in calf liver.小牛肝脏胆小管的一些结构特征。
Anat Rec. 1961 Jul;140:207-15. doi: 10.1002/ar.1091400306.

大鼠肝脏的质膜。富含胆小管的部分的分离及酶学特性分析。

Plasma membranes of the rat liver. Isolation and enzymatic characterization of a fraction rich in bile canaliculi.

作者信息

Song C S, Rubin W, Rifkind A B, Kappas A

出版信息

J Cell Biol. 1969 Apr;41(1):124-32. doi: 10.1083/jcb.41.1.124.

DOI:10.1083/jcb.41.1.124
PMID:4304740
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2107735/
Abstract

A method is described for the rapid isolation of a plasma membrane fraction containing a high concentration of intact bile canaliculi from the rat liver. Isolated bile canaliculi retain most of the ultrastructural features exhibited in the intact liver cell. The final fraction contains 5'-nucleotidase activity at approximately the same concentration as that in previous preparations of plasma membranes. In the presence of 0.01 M Mg(++), 5'-nucleotidase exhibits a double pH optimum at pH values of 7.5 and 9.5. The activities of glucose-6-phosphatase and alkaline phosphatase are present in low amounts. Cytochrome P-450 is not detectable. Na(+)-K(+)-activation of ATPase is observed to the extent of 20-36% in about half of the assays. The availability of a method for preparation of intact bile canaliculi should prove useful for studying the biochemical events associated with the transport of bile constituents into canaliculi.

摘要

本文描述了一种从大鼠肝脏中快速分离富含高浓度完整胆小管的质膜部分的方法。分离出的胆小管保留了完整肝细胞中呈现的大部分超微结构特征。最终部分中5'-核苷酸酶的活性浓度与先前制备的质膜中的大致相同。在0.01 M Mg(++)存在下，5'-核苷酸酶在pH值7.5和9.5时表现出双pH最佳值。葡萄糖-6-磷酸酶和碱性磷酸酶的活性含量较低。未检测到细胞色素P-450。在大约一半的测定中观察到Na(+)-K(+)对ATP酶的激活程度为20-36%。一种制备完整胆小管的方法的可用性应证明对研究与胆汁成分向胆小管转运相关的生化事件有用。