大鼠肝脏的质膜。富含胆小管的部分的分离及酶学特性分析。
Plasma membranes of the rat liver. Isolation and enzymatic characterization of a fraction rich in bile canaliculi.
作者信息
Song C S, Rubin W, Rifkind A B, Kappas A
出版信息
J Cell Biol. 1969 Apr;41(1):124-32. doi: 10.1083/jcb.41.1.124.
Abstract
A method is described for the rapid isolation of a plasma membrane fraction containing a high concentration of intact bile canaliculi from the rat liver. Isolated bile canaliculi retain most of the ultrastructural features exhibited in the intact liver cell. The final fraction contains 5'-nucleotidase activity at approximately the same concentration as that in previous preparations of plasma membranes. In the presence of 0.01 M Mg(++), 5'-nucleotidase exhibits a double pH optimum at pH values of 7.5 and 9.5. The activities of glucose-6-phosphatase and alkaline phosphatase are present in low amounts. Cytochrome P-450 is not detectable. Na(+)-K(+)-activation of ATPase is observed to the extent of 20-36% in about half of the assays. The availability of a method for preparation of intact bile canaliculi should prove useful for studying the biochemical events associated with the transport of bile constituents into canaliculi.
摘要
本文描述了一种从大鼠肝脏中快速分离富含高浓度完整胆小管的质膜部分的方法。分离出的胆小管保留了完整肝细胞中呈现的大部分超微结构特征。最终部分中5'-核苷酸酶的活性浓度与先前制备的质膜中的大致相同。在0.01 M Mg(++)存在下，5'-核苷酸酶在pH值7.5和9.5时表现出双pH最佳值。葡萄糖-6-磷酸酶和碱性磷酸酶的活性含量较低。未检测到细胞色素P-450。在大约一半的测定中观察到Na(+)-K(+)对ATP酶的激活程度为20-36%。一种制备完整胆小管的方法的可用性应证明对研究与胆汁成分向胆小管转运相关的生化事件有用。
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