Elsdale T, Foley R
J Cell Biol. 1969 Apr;41(1):298-311. doi: 10.1083/jcb.41.1.298.
Randomly seeded Petri dish cultures of embryonic human lung fibroblasts generate, in the course of their growth, highly ordered cellular arrangements. Thick, bilaterally symmetrical ridges with an axial polarity and an orthogonal, multilayered internal organization are observed within stationary cultures. The generation of these structures has been investigated. Ridges result from the spontaneous aggregation of cells in postconfluent cultures brought about by directed cell movements. These movements are promoted by the localized production of extracellular matrix sheets containing collagen, which provide new substrates for cellular colonization. Cells that have colonized one matrix substrate may secrete another above themselves, which will in turn be colonized. By a continuation of this cycle, thick stacks consisting of alternate layers of cells and matrix are produced to yield the observed aggregations. The distribution and shape of ridges in a culture imply that matrix substrates are confined to specific locations. The suggested control hypothesis assumes that all the cells in fibroblast cultures are potential producers of a single species of matrix. The serviceability of this matrix as a substrate for cellular colonization, however, is destroyed if the producer cells are motile. Matrix substrates, therefore, are only made by nonmotile cells.
随机接种的人胚胎肺成纤维细胞在培养过程中会形成高度有序的细胞排列。在静止培养物中可观察到具有轴向极性和正交多层内部结构的厚双侧对称脊。对这些结构的形成进行了研究。脊是由汇合后培养物中细胞的自发聚集产生的，这是由定向细胞运动引起的。这些运动由含有胶原蛋白的细胞外基质片的局部产生所促进，这些基质片为细胞定植提供了新的底物。已经定植在一个基质底物上的细胞可能会在自身上方分泌另一种基质，而这种基质又会被定植。通过这个循环的持续进行，会产生由细胞和基质交替层组成的厚堆叠，从而产生观察到的聚集。培养物中脊的分布和形状表明基质底物局限于特定位置。提出的控制假说假设成纤维细胞培养物中的所有细胞都是单一基质种类的潜在生产者。然而，如果产生基质的细胞是可移动的，那么这种基质作为细胞定植底物的可用性就会被破坏。因此，基质底物仅由不可移动的细胞产生。
