Smull C E, Ludwig E H
Appl Microbiol. 1969 Mar;17(3):449-53. doi: 10.1128/am.17.3.449-453.1969.
The plaque formation of poliovirus in HeLa cell monolayers was decreased upto 95% when the host cells were subcultured several times in a growth medium containing fresh bovine serum prior to their inoculation with virus. Extended incubation of infected cell monolayers indicated that the inhibition was in the form of a delay in plaque formation rather than a permanent inhibition of it. The inhibitory factor, which was found in most bovine sera, was very unstable and disappeared after storage of the serum at -20 C for a few weeks or at 36 C within a few days. In HeLa-cell monolayers, the fresh serum brought about a decrease in the plaque formation of all three poliovirus types as well as that of poliovirus ribonucleic acid. The plaque formation of poliovirus in monkey heart and HEp-2-cell monolayers was decreased irregularly by the use of fresh serum in the growth medium of these cells. Speculations were made as to the possible mode of action of the bovine serum inhibitor.
当宿主细胞在接种病毒前在含有新鲜牛血清的生长培养基中传代培养数次时，脊髓灰质炎病毒在HeLa细胞单层中的蚀斑形成减少了高达95%。对感染细胞单层的延长培养表明，这种抑制表现为蚀斑形成延迟，而非对其永久性抑制。在大多数牛血清中发现的抑制因子非常不稳定，在血清于-20℃储存几周后或在36℃放置几天后就会消失。在HeLa细胞单层中，新鲜血清导致所有三种脊髓灰质炎病毒类型以及脊髓灰质炎病毒核糖核酸的蚀斑形成减少。在这些细胞的生长培养基中使用新鲜血清，脊髓灰质炎病毒在猴心脏和HEp-2细胞单层中的蚀斑形成不规则减少。针对牛血清抑制剂可能的作用方式进行了推测。
