一种用于检测破碎膜中能量依赖性结构变化的荧光探针。
A fluorescence probe of energy-dependent structure changes in fragmented membranes.
作者信息
Azzi A, Chance B, Radda G K, Lee C P
出版信息
Proc Natl Acad Sci U S A. 1969 Feb;62(2):612-9. doi: 10.1073/pnas.62.2.612.
Abstract
The reaction of the fluorochrome, 8-anilino-1-naphthalene-sulfonic acid (ANS), with fragmented membranes from beef heart mitochondria has been studied. ANS fluorescence is found to be enhanced 25-fold on binding to the membrane fragments in the absence of energy conservation, and this enhancement is increased to 35-fold in the membrane energized by substrate plux oxygen. The fluorescence of bound ANS depends upon the energy state of the membrane fragments, as indicated by the effects of ATP, substrates of the respiratory chain, oligomycin, and uncouplers. It is concluded that the changes of ANS fluorescence indicate structural changes of the mitochondrial membrane associated with energy conservation. The time course of energization is readily followed by ANS, and has a half-time of two seconds at 26 degrees .
摘要
研究了荧光染料8-苯胺基-1-萘磺酸（ANS）与牛心线粒体破碎膜的反应。发现在没有能量守恒的情况下，ANS与膜片段结合时荧光增强25倍，而在由底物通量氧激发的膜中，这种增强增加到35倍。结合的ANS的荧光取决于膜片段的能量状态，如ATP、呼吸链底物、寡霉素和解偶联剂的作用所示。得出结论，ANS荧光的变化表明与能量守恒相关的线粒体膜结构变化。ANS很容易跟踪激发的时间进程，在26摄氏度时半衰期为两秒。
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