“拯救的”猿猴病毒40：在小鼠和人类细胞中提高转化效率
"Rescued" SV40: increased transforming efficiency in mouse and human cells.
作者信息
Todaro G J, Takemoto K K
出版信息
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1031-7. doi: 10.1073/pnas.62.4.1031.
Abstract
Plaque-size mutants of simian virus 40 (SV40) have been characterized with respect to their ability to transform mouse and human cells in vitro. Large plaque mutants of SV40 were extremely inefficient at transforming mouse or human cells, requiring approximately 50 times more infectious particles per transformation event than did the small plaque mutants. The minute plaque SV40 was the most efficient, requiring less than 10(3) plaque-forming units per transformation in mouse Balb/3T3 cells and only 1.5 x 10(4) plaque-forming units per transformation unit in the "susceptible" human strains. Both small and minute plaque virus were unable to help the large plaque virus to transform. Virus "rescued" from transformed 3T3 cells by cocultivation with monkey kidney cells was more efficient at transformation than the parental type. The increased efficiency could be shown for each plaque type and in human as well as in mouse cells. The property of "high efficiency transformation" is stable through at least two passages in monkey kidney cells.
摘要
猴病毒40（SV40）的噬菌斑大小突变体已根据其在体外转化小鼠和人类细胞的能力进行了表征。SV40的大噬菌斑突变体在转化小鼠或人类细胞方面效率极低，每次转化事件所需的感染性颗粒比小噬菌斑突变体多约50倍。微小噬菌斑SV40效率最高，在小鼠Balb/3T3细胞中每次转化所需的噬菌斑形成单位少于10³，在“易感”人类细胞系中每次转化单位仅需1.5×10⁴噬菌斑形成单位。小噬菌斑病毒和微小噬菌斑病毒都无法帮助大噬菌斑病毒进行转化。通过与猴肾细胞共培养从转化的3T3细胞中“拯救”出的病毒在转化方面比亲本类型更有效。每种噬菌斑类型在人类和小鼠细胞中都能表现出提高的效率。“高效转化”特性在猴肾细胞中至少传两代后仍保持稳定。
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