自发形成的和病毒产生的活杂种形成的定量比较。
A quantitative comparison of formation of spontaneous and virus-produced viable hybrids.
作者信息
Coon H G, Weiss M C
出版信息
Proc Natl Acad Sci U S A. 1969 Mar;62(3):852-9. doi: 10.1073/pnas.62.3.852.
Abstract
Ultraviolet-inactivated Sendai virus used in conjunction with a selective system reproducibly gives high yields of viable hybrid cell lines. With two different crosses, the frequency of hybrid colonies obtained after virus treatment was found to be 100 times greater than the frequency of spontaneous hybrids, and this increase was found to show little variation between 33 and 1000 hemagglutinating units of virus. No differences have been found between the properties of hybrids obtained after Sendai treatment and those obtained from untreated mixed cultures of parental cells.
摘要
与一种选择系统联合使用的紫外线灭活仙台病毒可重复性地产生高产量的活杂交细胞系。在两个不同的杂交实验中，发现病毒处理后获得的杂交菌落频率比自发杂交频率高100倍，并且发现这种增加在33至1000个血凝单位的病毒之间变化很小。经仙台处理后获得的杂交体与从未经处理的亲代细胞混合培养物中获得的杂交体的特性之间未发现差异。
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