Adams R G
J Lipid Res. 1969 Sep;10(5):473-8.
Phospholipids present in digitonin solutions of bovine rhodopsin have been identified and assayed. Digitonin interferes with extraction of lipids by the usual methods; digitonin was therefore removed from the preparation as an ergosterol digitonide, soluble in absolute ethanol but precipitated in 80% ethanol. The supernatant 80% ethanol contained one portion of the phospholipid, mainly choline and ethanolamine phosphoglycerides with traces of serine phosphoglyceride and sphingomyelin. The rhodopsin residue (free from digitonin) was extracted with chloroform-methanol 2:1; this extract contained the rest of the phospholipid, which consisted only of choline and ethanolamine phosphoglycerides. Plasmalogens were not found, but could have decomposed during the procedures.
已对牛视紫红质洋地黄皂苷溶液中的磷脂进行了鉴定和测定。洋地黄皂苷会干扰常规方法对脂质的提取；因此，洋地黄皂苷以洋地黄皂苷麦角甾醇的形式从制剂中去除，其可溶于无水乙醇，但在80%乙醇中沉淀。上清液80%乙醇中含有一部分磷脂，主要是胆碱和乙醇胺磷酸甘油酯，还有微量的丝氨酸磷酸甘油酯和鞘磷脂。视紫红质残余物（不含洋地黄皂苷）用氯仿 - 甲醇（2:1）提取；该提取物含有其余的磷脂，其仅由胆碱和乙醇胺磷酸甘油酯组成。未发现缩醛磷脂，但可能在操作过程中已分解。
