Giroux L, Verroust P, Morel-Maroger L, Delarue F, Delauche M, Sraer J D
Lab Invest. 1979 Apr;40(4):415-22.
The glomerular fibrinolytic activity (GFA) was measured during the development of glomerulonephritis induced by the intravenous injection of horse or rabbit antiglomerular basement membrane antibodies in rats either untreated or preimmunized with horse or rabbit Ig. A variety of nephritides was produced ranging from proteinuria with no or mild changes in glomerular architecture to severe diffuse proliferative glomerulonephritis with close to 100% crescent formation. GFA was measured on preparations of isolated glomeruli using a radioassay based on lysis of 125I fibrin adsorbed on a solid phase. In some experiments a fibrin plaque technique was also used. Both methods clearly demonstrated a marked increase in GFA with a good correlation between the two sets of results when the glomerular architecture is preserved. The increase in GFA is related both to the severity of the nephritis estimated by the percentage of glomeruli showing crescent formation and to the extent of the fibrin deposits. The results therefore indicate that the persistence of fibrin in the glomeruli and particularly in crescents is not due to a loss of GFA.
在大鼠中，通过静脉注射马或兔抗肾小球基底膜抗体诱导肾小球肾炎，在未处理或预先用马或兔免疫球蛋白免疫的大鼠中测量肾小球纤维蛋白溶解活性（GFA）。产生了多种肾炎，从蛋白尿且肾小球结构无变化或仅有轻微变化到严重的弥漫性增殖性肾小球肾炎，新月体形成接近100%。使用基于溶解吸附在固相上的125I纤维蛋白的放射测定法，在分离的肾小球制剂上测量GFA。在一些实验中还使用了纤维蛋白斑块技术。当肾小球结构得以保留时，两种方法均清楚地表明GFA显著增加，两组结果之间具有良好的相关性。GFA的增加既与通过显示新月体形成的肾小球百分比估计的肾炎严重程度有关，也与纤维蛋白沉积的程度有关。因此，结果表明纤维蛋白在肾小球尤其是新月体中的持续存在并非由于GFA的丧失。
