Raine C S, Bornstein M B
Lab Invest. 1979 Apr;40(4):423-32.
Mature myelinated cultures of mouse dorsal root ganglia (sometimes grown in combination with spinal cord tissue) have been exposed to unheated and heated (complement-inactivated) serum from rabbits with experimental allergic neuritis (EAN). Experimental cultures were examined for periods ranging from several hours to approximately 2 weeks of exposure. Some cultures were exposed to EAN serum for approximately 1 week then returned to normal medium for examination of the reversibility of the lesions. Unheated EAN serum induced demyelination of peripheral nervous system fibers within 96 hours of exposure. Following removal of the EAN serum, affected fibers remyelinated. Heated EAN serum produced a type of myelin swelling identical with that described previously in spinal cord explants exposed to serum from rabbits with experimental allergic encephalomyelitis. The lamellar spacing of the peripheral nervous system meylin was increased to approximately 23 nm. and the normal bifilar intraperiod line was increased to four leaflets. Some hypermyelination was seen. The swelling was incompletely reversible following removal of the heated serum. These findings are discussed in terms of their relevance to immunemediated demyelination and peripheral neuropathy associated with the hypergammaglobulinemic states.
小鼠背根神经节的成熟有髓培养物（有时与脊髓组织联合培养）已暴露于患有实验性变态反应性神经炎（EAN）的兔子的未加热和加热（补体灭活）血清中。对实验培养物进行了从数小时到约2周暴露时间的检查。一些培养物暴露于EAN血清约1周，然后恢复到正常培养基中以检查病变的可逆性。未加热的EAN血清在暴露96小时内诱导外周神经系统纤维脱髓鞘。去除EAN血清后，受影响的纤维重新髓鞘化。加热的EAN血清产生了一种髓鞘肿胀，与先前在暴露于患有实验性变态反应性脑脊髓炎的兔子血清的脊髓外植体中描述的肿胀相同。外周神经系统髓磷脂的板层间距增加到约23nm，正常的双丝状周期内线增加到四个小叶。可见一些髓鞘过度形成。去除加热血清后，肿胀不完全可逆。根据这些发现与免疫介导的脱髓鞘和与高球蛋白血症状态相关的周围神经病变的相关性进行了讨论。
