利用电子自旋共振光谱法观察体内对杀菌剂的反应。
Utilization of electron spin resonance spectroscopy for observation of in vivo response to fungicides.
作者信息
Rowlands J R, Gause E M
出版信息
Appl Microbiol. 1969 Oct;18(4):650-5. doi: 10.1128/am.18.4.650-655.1969.
Electron spin resonance studies of the in vivo response to fungicides (chloranil and thiram) by Aspergillus niger of different ages are described. The spores possessed a natural free radical signal which grew rapidly upon application of chloranil. Thiram, on the other hand, produced weak changes in the free radical region but easily detectable changes at lower fields, indicating involvement of copper and iron. In addition, flow experiments are described in which the production and decay of the chloranil semiquinone radical produced in the reaction of solutions of chloranil with extracts of bakers' yeast were monitored, both in the absence and in the presence of several buffers and respiratory chain inhibitors.
描述了对不同年龄的黑曲霉体内对杀菌剂（四氯苯醌和福美双）反应的电子自旋共振研究。孢子具有天然自由基信号，在施加四氯苯醌后迅速增强。另一方面，福美双在自由基区域产生微弱变化，但在较低磁场处有易于检测到的变化，表明铜和铁参与其中。此外，还描述了流动实验，其中监测了四氯苯醌溶液与面包酵母提取物反应中产生的四氯苯醌半醌自由基的生成和衰减，实验分别在不存在和存在几种缓冲液及呼吸链抑制剂的情况下进行。
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