辅酶A和酰基载体蛋白对兔肝果糖二磷酸酶的激活作用。
Activation of rabbit liver fructose diphosphatase by coenzyme A and acyl carrier protein.
作者信息
Nakashima K, Pontremoli S, Horecker B L
出版信息
Proc Natl Acad Sci U S A. 1969 Nov;64(3):947-51. doi: 10.1073/pnas.64.3.947.
Abstract
The catalytic activity of rabbit liver fructose diphosphatase is enhanced more than fourfold by treatment with coenzyme A or acyl carrier protein. Other sulfhydryl compounds, such as glutathione or cysteine, are without effect. Activation is reversed by reduced glutathione or cysteine, indicating that the enzyme and activator are linked by disulfide bridges. The activated enzyme derivative can be formed by a disulfide exchange reaction at alkaline pH or by an oxidation reaction which proceeds at neutral pH. The latter process requires O(2) and Cu(++), and is inhibited by EDTA.
摘要
用辅酶A或酰基载体蛋白处理后，兔肝果糖二磷酸酶的催化活性增强了四倍多。其他巯基化合物，如谷胱甘肽或半胱氨酸，则没有作用。还原型谷胱甘肽或半胱氨酸可逆转激活作用，这表明酶和激活剂是通过二硫键相连的。活化的酶衍生物可通过在碱性pH下的二硫键交换反应或在中性pH下进行的氧化反应形成。后一过程需要O₂和Cu²⁺，并受EDTA抑制。
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