来自藤黄微球菌的对紫外线照射的脱氧核糖核酸具有活性的核酸内切酶：纯化及性质
Endonuclease from Micrococcus luteus which has activity toward ultraviolet-irradiated deoxyribonucleic acid: purification and properties.
作者信息
Carrier W L, Setlow R B
出版信息
J Bacteriol. 1970 Apr;102(1):178-86. doi: 10.1128/jb.102.1.178-186.1970.
Abstract
An endonuclease purified approximately 3,200-fold from Micrococcus luteus is active on native ultraviolet-irradiated deoxyribonucleic acid (DNA), but is inactive on unirradiated native or denatured DNA and has no activity toward irradiated denatured DNA. The major type of lesion for the nucleolytic activity is the cyclobutane pyrimidine dimer. The enzyme makes a number of single-strand breaks approximately equal to the number of dimers, but dimers are not excised. This endonuclease-a small molecular weight protein-therefore has all the attributes hypothesized for the first enzyme in the sequential steps in repair of DNA in vivo. Another paper shows that the endonuclease is able to reactivate ultraviolet-irradiated transforming DNA.
摘要
一种从藤黄微球菌中纯化出来、纯化倍数约为3200倍的核酸内切酶，对天然的紫外线照射的脱氧核糖核酸（DNA）有活性，但对未照射的天然或变性DNA无活性，且对照射过的变性DNA也没有活性。核酸水解活性的主要损伤类型是环丁烷嘧啶二聚体。该酶产生的单链断裂数量与二聚体数量大致相等，但二聚体并未被切除。因此，这种核酸内切酶——一种小分子量蛋白质——具有体内DNA修复连续步骤中第一种酶所假设的所有特性。另一篇论文表明，该核酸内切酶能够使紫外线照射的转化DNA重新激活。
相似文献
1
Endonuclease from Micrococcus luteus which has activity toward ultraviolet-irradiated deoxyribonucleic acid: purification and properties.来自藤黄微球菌的对紫外线照射的脱氧核糖核酸具有活性的核酸内切酶：纯化及性质
J Bacteriol. 1970 Apr;102(1):178-86. doi: 10.1128/jb.102.1.178-186.1970.
2
Endonuclease from Micrococcus luteus which has activity toward ultraviolet-irradiated deoxyribonucleic acid: its action on transforming deoxyribonucleic acid.来自藤黄微球菌的对紫外线照射的脱氧核糖核酸具有活性的核酸内切酶：其对转化脱氧核糖核酸的作用。
J Bacteriol. 1970 Apr;102(1):187-92. doi: 10.1128/jb.102.1.187-192.1970.
3
In vivo role of the UV-endonuclease from Micrococcus luteus in the repair of DNA.藤黄微球菌紫外线内切核酸酶在DNA修复中的体内作用
Nat New Biol. 1971 Nov 10;234(45):47-50. doi: 10.1038/newbio234047a0.
4
Repair of ultraviolet-damaged DNA in Micrococcus lysodeikticus. I. An endonuclease specific for ultraviolet-irradiated DNA.溶壁微球菌中紫外线损伤DNA的修复。I. 一种对紫外线照射的DNA具有特异性的核酸内切酶。
Biochim Biophys Acta. 1971 Jan 1;228(1):67-82. doi: 10.1016/0005-2787(71)90547-8.
5
Purification and characterization of an endonuclease from Micrococcus luteus that acts on depurinated and carcinogen-modified DNA.从藤黄微球菌中纯化和鉴定一种作用于脱嘌呤和致癌物修饰DNA的核酸内切酶。
Eur J Biochem. 1978 Sep 1;89(2):607-18. doi: 10.1111/j.1432-1033.1978.tb12565.x.
6
7
Enzymatic repair of DNA, 1. Purification of two enzymes involved in the excision of thymine dimers from ultraviolet-irradiated DNA.DNA的酶促修复，1. 从紫外线照射的DNA中切除胸腺嘧啶二聚体所涉及的两种酶的纯化。
Proc Natl Acad Sci U S A. 1969 May;63(1):144-51. doi: 10.1073/pnas.63.1.144.
8
9
Endonucleolytic activity from Micrococcus luteus that acts on -ray-induced damage in plasmid DNA of Escherichia coli minicells.藤黄微球菌的核酸内切酶活性作用于大肠杆菌微小细胞质粒DNA中的γ射线诱导损伤。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2927-31. doi: 10.1073/pnas.69.10.2927.
10
Enzymes involved in the early stages of repair of ultraviolet-irradiated DNA.参与紫外线照射DNA修复早期阶段的酶。
Cold Spring Harb Symp Quant Biol. 1968;33:229-34. doi: 10.1101/sqb.1968.033.01.026.
引用本文的文献
1
Batch effects account for the main findings of an in utero human intestinal bacterial colonization study.批量效应解释了一项子宫内人类肠道细菌定植研究的主要发现。
Microbiome. 2021 Jan 12;9(1):6. doi: 10.1186/s40168-020-00949-z.
2
Participation of the intracellular enzymes in the control of mutational processes : Part 4: the role of UV-specific endonuclease and medium composition in the induction of genic mutations in Escherichia coli.参与细胞内酶对突变过程的控制：第 4 部分：UV 特异性内切酶和培养基组成在诱导大肠杆菌基因突变中的作用。
Theor Appl Genet. 1979 May;55(3-4):119-28. doi: 10.1007/BF00295437.
3
Repair of 2 um Plasmid DNA in Saccharomyces cerevisiae.酵母 2μm 质粒 DNA 的修复。
Curr Genet. 1980 Dec;2(3):207-10. doi: 10.1007/BF00435687.
4
qUVR-10, a major quantitative trait locus for ultraviolet-B resistance in rice, encodes cyclobutane pyrimidine dimer photolyase.qUVR-10是水稻中一个主要的抗紫外线B定量性状基因座，编码环丁烷嘧啶二聚体光解酶。
Genetics. 2005 Dec;171(4):1941-50. doi: 10.1534/genetics.105.044735. Epub 2005 Jun 18.
5
Photoreactivation of Escherichia coli after low- or medium-pressure UV disinfection determined by an endonuclease sensitive site assay.通过核酸内切酶敏感位点分析测定低或中压紫外线消毒后大肠杆菌的光复活作用。
Appl Environ Microbiol. 2002 Dec;68(12):6029-35. doi: 10.1128/AEM.68.12.6029-6035.2002.
6
Efficacy of UV irradiation in inactivating Cryptosporidium parvum oocysts.紫外线照射对灭活微小隐孢子虫卵囊的效果。
Appl Environ Microbiol. 2002 Nov;68(11):5387-93. doi: 10.1128/AEM.68.11.5387-5393.2002.
7
Determination of pyrimidine dimers in Escherichia coli and Cryptosporidium parvum during UV light inactivation, photoreactivation, and dark repair.紫外线灭活、光复活和暗修复过程中大肠杆菌和微小隐孢子虫嘧啶二聚体的测定
Appl Environ Microbiol. 2001 Oct;67(10):4630-7. doi: 10.1128/AEM.67.10.4630-4637.2001.
8
UV radiation-sensitive norin 1 rice contains defective cyclobutane pyrimidine dimer photolyase.对紫外线辐射敏感的诺林1号水稻含有缺陷的环丁烷嘧啶二聚体光裂合酶。
Plant Cell. 2000 Sep;12(9):1569-78. doi: 10.1105/tpc.12.9.1569.
9
UV endonuclease of Micrococcus luteus, a cyclobutane pyrimidine dimer-DNA glycosylase/abasic lyase: cloning and characterization of the gene.藤黄微球菌的紫外线核酸内切酶，一种环丁烷嘧啶二聚体-DNA糖基化酶/无碱基裂合酶：基因的克隆与特性分析
Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):593-8. doi: 10.1073/pnas.94.2.593.
10
Inducible removal of UV-induced pyrimidine dimers from transcriptionally active and inactive genes of Saccharomyces cerevisiae.从酿酒酵母转录活性和非活性基因中诱导去除紫外线诱导的嘧啶二聚体。
Mol Gen Genet. 1993 May;239(1-2):28-32. doi: 10.1007/BF00281597.
本文引用的文献
1
Nonphotoreactivating Repair of Ultraviolet Light-Damaged Transforming Deoxyribonucleic Acid by Micrococcus lysodeikticus Extracts.溶壁微球菌提取物对紫外线损伤的转化脱氧核糖核酸的非光复活修复
J Bacteriol. 1965 Sep;90(3):681-6. doi: 10.1128/jb.90.3.681-686.1965.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
BIOCHEMICAL AND GENETIC STUDIES OF INTEGRATION AND RECOMBINATION IN BACILLUS SUBTILIS TRANSFORMATION.枯草芽孢杆菌转化中整合与重组的生化及遗传学研究
Genetics. 1964 Oct;50(4):717-38. doi: 10.1093/genetics/50.4.717.
4
DARK REACTIVATION OF ULTRAVIOLET IRRADIATED BACTERIOPHAGE DEOXYRIBONUCLEIC ACID IN VITRO.紫外线照射的噬菌体脱氧核糖核酸在体外的暗复活作用
Biochim Biophys Acta. 1964 Feb 17;80:346-8.
5
Differential destruction of the transforming activity of damaged deoxyribonucleic acid by a bacterial enzyme.一种细菌酶对受损脱氧核糖核酸转化活性的差异性破坏。
Proc Natl Acad Sci U S A. 1962 Sep 15;48(9):1670-5. doi: 10.1073/pnas.48.9.1670.
6
Peptides obtained by tryptic hydrolysis of performic acid-oxidized ribonuclease.通过对过甲酸氧化的核糖核酸酶进行胰蛋白酶水解得到的肽段。
J Biol Chem. 1956 Apr;219(2):623-42.
7
An endonucleolytic activity specific for ultraviolet-irradiated DNA in wild type and mutant strains of Micrococcus lysodeikticus.溶壁微球菌野生型和突变株中对紫外线照射的DNA具有特异性的核酸内切酶活性。
Biochem Biophys Res Commun. 1967 Jun 9;27(5):539-45. doi: 10.1016/s0006-291x(67)80021-4.
8
Studies on the yeast photoreactivating enzyme. I. A method for the large scale purification and some properties of the enzyme.酵母光复活酶的研究。I. 一种大规模纯化该酶的方法及其某些特性。
J Biol Chem. 1966 Jan 25;241(2):516-23.
9
Cyclobutane-type pyrimidine dimers in polynucleotides.多核苷酸中的环丁烷型嘧啶二聚体。
Science. 1966 Jul 22;153(3734):379-86. doi: 10.1126/science.153.3734.379.
10
The chemical nature of photoreactivable lesions in DNA.DNA中光可修复损伤的化学性质。
Proc Natl Acad Sci U S A. 1965 Jun;53(6):1430-6. doi: 10.1073/pnas.53.6.1430.
Zotero 插件