Keudell K C, Goldberg H S
Appl Microbiol. 1970 Mar;19(3):505-11. doi: 10.1128/am.19.3.505-511.1970.
Enzyme patterns were obtained by starch-gel electrophoresis of cell-free extracts from organisms in the family Bacteroidaceae. Esterases, phosphatases, and lactic and succinic dehydrogenases were detected but offered no possibility for classification purposes. Alpha-glycerophosphate dehydrogenase and 6-phosphogluconate dehydrogenase were not detected. Zymograms of malic, glutamic, and glucose-6-phosphate dehydrogenases separated Bacteroides species from Sphaerophorus and Fusobacterium species. Malic dehydrogenase and glucose-6-phosphate dehydrogenase were found in Bacteroides but not Sphaerophorus and Fusobacterium. These dehydrogenase zymograms placed three gram-negative, nonsporeforming anaerobic rod isolates with the Bacteroides species. A close correlation was found between the classification of Bacteroidaceae by zymogram analysis and a numerical taxonomy scheme previously published from this laboratory.
通过对拟杆菌科生物的无细胞提取物进行淀粉凝胶电泳获得酶谱。检测到了酯酶、磷酸酶以及乳酸脱氢酶和琥珀酸脱氢酶，但这些酶对于分类目的没有提供可能性。未检测到α-甘油磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶。苹果酸脱氢酶、谷氨酸脱氢酶和6-磷酸葡萄糖脱氢酶的酶谱图将拟杆菌属物种与球形梭菌属和梭杆菌属物种区分开来。在拟杆菌属中发现了苹果酸脱氢酶和6-磷酸葡萄糖脱氢酶，而在球形梭菌属和梭杆菌属中未发现。这些脱氢酶酶谱图将三株革兰氏阴性、无芽孢厌氧杆状分离株归为拟杆菌属物种。通过酶谱分析对拟杆菌科进行的分类与本实验室先前发表的数值分类方案之间发现了密切的相关性。
