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乳酸脱氢酶的生理浓度与底物抑制作用。

Physiological concentrations of lactate dehydrogenases and substrate inhibition.

作者信息

Everse J, Berger R L, Kaplan N O

出版信息

Science. 1970 Jun 5;168(3936):1236-8. doi: 10.1126/science.168.3936.1236.

DOI:10.1126/science.168.3936.1236
PMID:4315228
Abstract

Lactate dehydrogenases at physiological concentrations are inhibited by high concentrations of pyruvate when the enzyme and the pyruvate are incubated in the presence of oxidized nicotinamide-adenine dinucleotide before assay. The inhibition is much more pronounced with the H-type than with the M-type lactate dehydrogenase. These results suggest that substrate inhibition may be operative in vivo.

摘要

当在测定前将酶和丙酮酸在氧化型烟酰胺腺嘌呤二核苷酸存在的情况下孵育时，生理浓度的乳酸脱氢酶会受到高浓度丙酮酸的抑制。与M型乳酸脱氢酶相比，H型乳酸脱氢酶的抑制作用更为明显。这些结果表明底物抑制可能在体内起作用。

相似文献

1
Physiological concentrations of lactate dehydrogenases and substrate inhibition.乳酸脱氢酶的生理浓度与底物抑制作用。
Science. 1970 Jun 5;168(3936):1236-8. doi: 10.1126/science.168.3936.1236.
2
Lactate dehydrogenase isozymes: further kinetic studies at high enzyme concentration.乳酸脱氢酶同工酶：在高酶浓度下的进一步动力学研究。
Science. 1970 Jul 31;169(3944):480-1. doi: 10.1126/science.169.3944.480.
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In vitro inhibition of lactate dehydrogenase by insecticidal polychlorinated hydrocarbons. I. Mechanism of inhibition: possible association of reduced nicotinamide adenine dinucleotide with Mirex.杀虫性多氯代烃对乳酸脱氢酶的体外抑制作用。I. 抑制机制：还原型烟酰胺腺嘌呤二核苷酸与灭蚁灵的可能结合
J Agric Food Chem. 1976 Mar-Apr;24(2):241-4. doi: 10.1021/jf60204a027.
4
Bimodal substrate inhibition of lactate dehydrogenase. Factors affecting the enzyme in vivo.
Biochemistry. 1978 May 2;17(9):1646-53. doi: 10.1021/bi00602a011.
5
The nature of the substrate inhibition in lactate dehydrogenases as studied by a spin-labeled derivative of NAD.通过烟酰胺腺嘌呤二核苷酸的自旋标记衍生物研究乳酸脱氢酶中的底物抑制性质。
Biochim Biophys Acta. 1979 Mar 16;567(1):49-59. doi: 10.1016/0005-2744(79)90171-2.
6
Identification of lactate dehydrogenase isoenzymes by rapid kinetics.通过快速动力学鉴定乳酸脱氢酶同工酶
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1761-5. doi: 10.1073/pnas.69.7.1761.
7
H4-isozyme of lactate dehydrogenase in the solution of sodium chloride--3. The enzymatic activity and the pyruvate inhibition.氯化钠溶液中乳酸脱氢酶的H4同工酶——3. 酶活性与丙酮酸抑制作用
Int J Biochem. 1983;15(2):185-90. doi: 10.1016/0020-711x(83)90064-2.
8
[Inhibition of lactate dehydrogenase by nicotinamide adenine dinucleotide].
Hoppe Seylers Z Physiol Chem. 1968 Mar;349(3):293-6.
9
Inhibition of human erythrocyte lactate dehydrogenase by high concentrations of pyruvate. Evidence for the competitive substrate inhibition.
Eur J Biochem. 1977 Sep;78(2):569-74. doi: 10.1111/j.1432-1033.1977.tb11770.x.
10
Substrate inhibition of soluble and bound lactate dehydrogenase (isoenzyme 5).可溶性和结合型乳酸脱氢酶（同工酶5）的底物抑制作用
Arch Biochem Biophys. 1973 Jan;154(1):471-5. doi: 10.1016/0003-9861(73)90080-5.

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