一种用于检测透明质酸酶活性的新型微量测定法。
A new micro-assay for hyaluronidase activity.
作者信息
Chen S S, Hsu D S, Hoffman P
出版信息
Clin Chim Acta. 1979 Jul 16;95(2):277-84. doi: 10.1016/0009-8981(79)90369-3.
A new micro-method for the assay of hyaluronidase activity is described. The method utilizes chondroitin sulfate as a substrate. The degraded products of chondroitin sulfate by hyaluronidase are determined by modified disc gel electrophoresis. The products are first concentrated into a single band in acrylamide gel, and then the band is stained with cetylpyridinium chloride. The absorbance of the band is proportional to the amount of the degraded products. The hyaluronidase activity is therefore linearly related to the absorbance. This procedure represents a sensitive method for the assay of hyaluronidase activity in the serum and urine.
本文描述了一种测定透明质酸酶活性的新微方法。该方法利用硫酸软骨素作为底物。通过改良的圆盘凝胶电泳测定透明质酸酶作用于硫酸软骨素的降解产物。产物首先在丙烯酰胺凝胶中浓缩成一条带，然后用十六烷基吡啶氯化物对该带进行染色。带的吸光度与降解产物的量成正比。因此，透明质酸酶活性与吸光度呈线性相关。该程序是一种测定血清和尿液中透明质酸酶活性的灵敏方法。
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