无细胞马立克氏病疱疹病毒和一种来自火鸡的疱疹病毒的冻干处理
Lyophilization of cell-free Marek's disease herpesvirus and a herpesvirus from turkeys.
作者信息
Calnek B W, Hitchner S B, Adldinger H K
出版信息
Appl Microbiol. 1970 Nov;20(5):723-6. doi: 10.1128/am.20.5.723-726.1970.
Abstract
Cell extracts of the JM and GA strains of Marek's disease herpesvirus and the FC 126 strain of turkey herpesvirus were lyophilized with various stabilizers. Much higher virus titers were obtained with stabilizer than without stabilizer. Titers increased even further in the case of the Marek's disease virus strains by the addition of a chelating agent, disodium ethylenediaminetetraacetate.
摘要
马立克氏病疱疹病毒的JM和GA毒株以及火鸡疱疹病毒的FC 126毒株的细胞提取物用各种稳定剂进行冻干处理。使用稳定剂获得的病毒滴度比不使用稳定剂时高得多。对于马立克氏病病毒毒株，通过添加螯合剂乙二胺四乙酸二钠，滴度进一步提高。
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